Evolving Techniques For In Vitro Somatic Embryogenesis In Polyembryonic Mango Varieties
By: Ramesh P.
Contributor(s): Rajmohan K(Guide).
Material type:
BookPublisher: Vellayani Department of Horticulture, College of Agriculture 1998DDC classification: 635 Online resources: Click here to access online | Click here to access online Dissertation note: MSc Abstract: Studies were conducted for evolving techniques for in vitro somatic
embryogenesis in polyembryonic mango varieties during 1994 - 1996 in the
Plant Tissue Culture Laboratory of the Department of Horticulture, College of
Agriculture, Vellayani. Attempts were made to standardise the various stages
of somatic embryogenesis, namely, induction, initiation, maturation and
germination using nucellus and embryo mass as explants.
Among the SIX varieties attempted for induction of somatic
embryogenesis from nucellus, Kalluvarikka recorded the highest percentage of
response (85.0) and Vellari Manga the least (68.9).
Induction of somatic embryogenesis from nucellus was found to occur
at its maximum on MS medium having half strength major salts, supplemented
with 2,4 - D 5.0 mg/l, GA3 5.0 mg/l, glutamine 400 mg/l, sucrose 60 g/l,
coconut water 200 ml/l, agar 6.0 g/l and activated charcoal 2.5 g/l, in
darkness. The highest callus index was recorded when 2,4 - D 5.0 mg/l was
substituted with BA 16 mg/l in the same medium.
Initation of .somatic embryo genesis from nucellus occurred at its best in
darkness on MS medium having half strength major salts, supplemented with
BA 1.0 mg/l, glutamine 400 mg/l, casein hydrolysate 500 mg/I, sucrose 60 g/l,
coconut water 200 mIll agar 5.5 g/l and activated charcoal 2.5 g/l.
Maturation of nucellus derived somatic embryoids was found at its best
on a combination of basal media with B-5 major salts and MS minor salts
supplemented with ABA 3.0 mg/l, casein hydrolysate 100 mg/l, sucrose 40 g/l,
PVP 10 g/l, coconut water 200 ml/l and agar 5.0 g/l, in darkness.
Germination of somatic embryoids derived from nucellus occurred only
in presence of light, on a combination of basal media with B-5 major salts, MS
minor salts, supplemented with BA 0.1 mg/l, sucrose 50 g/l, PVP 10 g/1 and
agar 5.0 g/l.
Somatic embryos were produced directly from embryo mass explants in
darkness on solid MS medium having half strength of major salts supplemented
with 2,4 - D 5.0 mg/l, GA3 5.0 mg/l, glutamine 400 mg/l, sucrose 60 g/l,
coconut water 200 ml/l, agar 6.0 g/I and activated charcoal 2.5 g/l. The
embryoids having sufficient size resulted from this treatment were able to
attain near normal germination in presence of light on medium having a
combination of B - 5 major salts and MS minor salts, supplemented with BA
0.1 mg/l or 0.2 mg/l, sucrose 50 g/l, PVP 10 g/l and agar 5.0 g/1.
| Item type | Current location | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|
Theses
|
KAU Central Library, Thrissur Theses | 635 RAM/EV (Browse shelf) | Available | 171366 |
MSc
Studies were conducted for evolving techniques for in vitro somatic
embryogenesis in polyembryonic mango varieties during 1994 - 1996 in the
Plant Tissue Culture Laboratory of the Department of Horticulture, College of
Agriculture, Vellayani. Attempts were made to standardise the various stages
of somatic embryogenesis, namely, induction, initiation, maturation and
germination using nucellus and embryo mass as explants.
Among the SIX varieties attempted for induction of somatic
embryogenesis from nucellus, Kalluvarikka recorded the highest percentage of
response (85.0) and Vellari Manga the least (68.9).
Induction of somatic embryogenesis from nucellus was found to occur
at its maximum on MS medium having half strength major salts, supplemented
with 2,4 - D 5.0 mg/l, GA3 5.0 mg/l, glutamine 400 mg/l, sucrose 60 g/l,
coconut water 200 ml/l, agar 6.0 g/l and activated charcoal 2.5 g/l, in
darkness. The highest callus index was recorded when 2,4 - D 5.0 mg/l was
substituted with BA 16 mg/l in the same medium.
Initation of .somatic embryo genesis from nucellus occurred at its best in
darkness on MS medium having half strength major salts, supplemented with
BA 1.0 mg/l, glutamine 400 mg/l, casein hydrolysate 500 mg/I, sucrose 60 g/l,
coconut water 200 mIll agar 5.5 g/l and activated charcoal 2.5 g/l.
Maturation of nucellus derived somatic embryoids was found at its best
on a combination of basal media with B-5 major salts and MS minor salts
supplemented with ABA 3.0 mg/l, casein hydrolysate 100 mg/l, sucrose 40 g/l,
PVP 10 g/l, coconut water 200 ml/l and agar 5.0 g/l, in darkness.
Germination of somatic embryoids derived from nucellus occurred only
in presence of light, on a combination of basal media with B-5 major salts, MS
minor salts, supplemented with BA 0.1 mg/l, sucrose 50 g/l, PVP 10 g/1 and
agar 5.0 g/l.
Somatic embryos were produced directly from embryo mass explants in
darkness on solid MS medium having half strength of major salts supplemented
with 2,4 - D 5.0 mg/l, GA3 5.0 mg/l, glutamine 400 mg/l, sucrose 60 g/l,
coconut water 200 ml/l, agar 6.0 g/I and activated charcoal 2.5 g/l. The
embryoids having sufficient size resulted from this treatment were able to
attain near normal germination in presence of light on medium having a
combination of B - 5 major salts and MS minor salts, supplemented with BA
0.1 mg/l or 0.2 mg/l, sucrose 50 g/l, PVP 10 g/l and agar 5.0 g/1.
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