Plasmid Profile Of avian Strains Of Pasteurella Multocida
By: Balakrishnan G.
Contributor(s): Mini, M(Guide).
Material type:
BookPublisher: Mannuthy Department of Microbiology, College of Veterinary and Animal Sciences 1998DDC classification: 636.089 6 Online resources: Click here to access online | Click here to access online Dissertation note: MVSc Abstract: Four isolates of P. multocida (DCl, DC2, FCl and FC2) from
avian species (chicken and ducks) were subj ected to various
typing methods like biotyping, antibiogram and plasmid
profiling. The pathogenicity of the isolates was also
ascertained in mice, rabbits chicken and ducks.
By biotyping, the isolates were found to belong to two
subspecies P. mul tocida subsp. mul tocida and P. mul t.oc i cie
subsp. septica. All the isolates fermented xylose and none of
them fermented dulcitol.
Antibiogram of the isolates was carried out and they wer~
found to be resistant to furazolidone, metronidazole anC
nalidixic acid. Some of the isolates are also resistant tc
sulpha and trimethoprim.
All the isolates were pathogenic to mice on rip injection.
Duck isolates (DCl and DC2) were found to be pathogenic to
rabbits killing them on fourth and fifth day respectively.
The isolates DCl and DC2 were lethal to orally infected
chicks within 48 h of administration and P. multocida could e
reisolated from these birds. The same isolates killed the SiC
injected ducks within 24 and 48 h.
11
The chicken isolates obtained from apparently healthy birds
were non pathogenic to rabbits, chicks and ducks.
Plasmid profiling revealed the presence of plasmids in
three of the four isolates screened. One isolate was plasmid
less and another isolate contained five plasmids. The molecular
size of the plasmids ranged from 2.69 kb to 7.07 kb. One
isolate did not contain plasmids at all. An isolate (FC2) which
was not pathogenic to rabbits, chicks and ducks contained
plasmids. Isolate FCl which was resistant to six antibiotics
did not possess plasmids. Hence plasmids obtained in this study
may not be associated with either antibiotic resistance or
pathogenicity in experimental animals.
A combination of two parameters such as plasmid profile and
one of the typing methods, biotyping or antibiogram made it
possible to differentiate the isolates. Thus, the plasmid
profile analysis with anyone of the typing methods could be
used as an epidemiological tool in the differentiation of
P. multocida strains of avian origin.
| Item type | Current location | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|
Theses
|
KAU Central Library, Thrissur Theses | 636.089 6 BAL/PL (Browse shelf) | Available | 171411 |
MVSc
Four isolates of P. multocida (DCl, DC2, FCl and FC2) from
avian species (chicken and ducks) were subj ected to various
typing methods like biotyping, antibiogram and plasmid
profiling. The pathogenicity of the isolates was also
ascertained in mice, rabbits chicken and ducks.
By biotyping, the isolates were found to belong to two
subspecies P. mul tocida subsp. mul tocida and P. mul t.oc i cie
subsp. septica. All the isolates fermented xylose and none of
them fermented dulcitol.
Antibiogram of the isolates was carried out and they wer~
found to be resistant to furazolidone, metronidazole anC
nalidixic acid. Some of the isolates are also resistant tc
sulpha and trimethoprim.
All the isolates were pathogenic to mice on rip injection.
Duck isolates (DCl and DC2) were found to be pathogenic to
rabbits killing them on fourth and fifth day respectively.
The isolates DCl and DC2 were lethal to orally infected
chicks within 48 h of administration and P. multocida could e
reisolated from these birds. The same isolates killed the SiC
injected ducks within 24 and 48 h.
11
The chicken isolates obtained from apparently healthy birds
were non pathogenic to rabbits, chicks and ducks.
Plasmid profiling revealed the presence of plasmids in
three of the four isolates screened. One isolate was plasmid
less and another isolate contained five plasmids. The molecular
size of the plasmids ranged from 2.69 kb to 7.07 kb. One
isolate did not contain plasmids at all. An isolate (FC2) which
was not pathogenic to rabbits, chicks and ducks contained
plasmids. Isolate FCl which was resistant to six antibiotics
did not possess plasmids. Hence plasmids obtained in this study
may not be associated with either antibiotic resistance or
pathogenicity in experimental animals.
A combination of two parameters such as plasmid profile and
one of the typing methods, biotyping or antibiogram made it
possible to differentiate the isolates. Thus, the plasmid
profile analysis with anyone of the typing methods could be
used as an epidemiological tool in the differentiation of
P. multocida strains of avian origin.
There are no comments for this item.