Micropropagation In Selected Varieties Of Anthurium Andreanum Lind.
By: Anitha Susan Thomas.
Contributor(s): Ramachandran Nair S(Guide).
Material type:
BookPublisher: Vellayani Department of Horticulture, College of Agriculture 1996Description: 97p.DDC classification: 635 Online resources: Click here to access online | Click here to access online Dissertation note: MSc. Abstract: Studies were conducted to optimise the
in vitro
propagation
techniques
via somatic
organogenesis
in
Anthuruim andreanum va~ieties (Dragon's Tongue, Flaking, Pompon Red
Honeymoon Red and Nitta) during 1994-1995 at the Department
of Horticulture, College of Agriculture, Vellayani.
All the five varieties responded to the callusing
treatments in varying degrees. Regeneration was obtained only
in the variety Dragon's Tongue and this variety was
subjected
to different treatments for
refinement
of
callusing and shoot proliferation. The protocol for in vitro
propagation of the variety Dragon's Tongue could be
standardised.
l\mong the different explants tried only leaf
explants were found responsive for callusing. Callus was
best initiated (50.0 % ) within 60 days when leaf explants
were cultured in darkness on modified Murashige and Skoog
basal medium (NH4N03 200 mg/l) supplemented with 2,4-D 0.5
mg/l, BA 0.5 mg/l, sucrose 30.0 g/l and agar 6.0 g/l.
The callus cultures were subcultured in the same
medium for two months for callus multiplication.
Regeneration was obtained within one month on
Murashige and Skoog basal medium supplemented with BA 0.5
mg/l, IAA 2.0 mg/l, sucrose 30.0 g/l and agar 6.0 g/l. Light
was essential for regeneration.
The
shoots) was
supplemented
hydrolysate
maximum rate of shoot proliferation (13.49
observed on Murashige and Skoog basal medium
with kinetin 1.5 mg/l, IAA 3.0 mg/l, casein
150.0 mg/l, sucrose 30.0 g/l and agar 6.0 g/l
after a period of six weeks.
Improvement in growth of shoots was obtained by
culturing in Murashige and Skoog basal medium supplemented
with activated charcoal (1.0 g/l) and further subculturing
to Murashige and Skoog basal medium supplemented with
kinetin 0.5 mg/l and IAA 16.0 mg/l.
A separate rooting phase was not necessary since
satisfactory rooting was obtained in the shoot proliferation
medium itself.
Rooted plantlets gave a survival rate of 60.0 per
cent on planting out.
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|---|---|---|---|---|---|
Theses
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KAU Central Library, Thrissur Theses | 635 ANI/MI (Browse shelf) | Available | 171420 |
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MSc.
Studies were conducted to optimise the
in vitro
propagation
techniques
via somatic
organogenesis
in
Anthuruim andreanum va~ieties (Dragon's Tongue, Flaking, Pompon Red
Honeymoon Red and Nitta) during 1994-1995 at the Department
of Horticulture, College of Agriculture, Vellayani.
All the five varieties responded to the callusing
treatments in varying degrees. Regeneration was obtained only
in the variety Dragon's Tongue and this variety was
subjected
to different treatments for
refinement
of
callusing and shoot proliferation. The protocol for in vitro
propagation of the variety Dragon's Tongue could be
standardised.
l\mong the different explants tried only leaf
explants were found responsive for callusing. Callus was
best initiated (50.0 % ) within 60 days when leaf explants
were cultured in darkness on modified Murashige and Skoog
basal medium (NH4N03 200 mg/l) supplemented with 2,4-D 0.5
mg/l, BA 0.5 mg/l, sucrose 30.0 g/l and agar 6.0 g/l.
The callus cultures were subcultured in the same
medium for two months for callus multiplication.
Regeneration was obtained within one month on
Murashige and Skoog basal medium supplemented with BA 0.5
mg/l, IAA 2.0 mg/l, sucrose 30.0 g/l and agar 6.0 g/l. Light
was essential for regeneration.
The
shoots) was
supplemented
hydrolysate
maximum rate of shoot proliferation (13.49
observed on Murashige and Skoog basal medium
with kinetin 1.5 mg/l, IAA 3.0 mg/l, casein
150.0 mg/l, sucrose 30.0 g/l and agar 6.0 g/l
after a period of six weeks.
Improvement in growth of shoots was obtained by
culturing in Murashige and Skoog basal medium supplemented
with activated charcoal (1.0 g/l) and further subculturing
to Murashige and Skoog basal medium supplemented with
kinetin 0.5 mg/l and IAA 16.0 mg/l.
A separate rooting phase was not necessary since
satisfactory rooting was obtained in the shoot proliferation
medium itself.
Rooted plantlets gave a survival rate of 60.0 per
cent on planting out.
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