Comparative Efficiency Of Different Vaccines Against Pasteurellosis In Ducks
By: Jayakumar, P S.
Contributor(s): Jayaprakasan V(Guide).
Material type:
BookPublisher: Mannuthy Department of Microbiology, College of Veterinary and Animal Sciences 1998DDC classification: 636.089 6 Online resources: Click here to access online | Click here to access online Dissertation note: MVSc Abstract: Two isolates of Pasteurella mul tocida from ducks viz. DP1
and DP5 isolated from ailing/dead ducks from Kuttanad area and
maintained in the virulent form were used separately for the
preparation of different vaccines viz., bacterin, bacterin
with oil adjuvant and sonicated antigen with oil adjuvant.
The biological and biochemical characters of both the
isolates were compared and are in confirmity with the
characters described for the species by earlier workers.
Both the isolates were pathogenic to ducks and mice as
all the inoculated ducks and mice were killed with in 72 h.
The LDso for the DP1 isolate in one month old ducklings and six
month old ducks were determined to be 23 and 32 cells
respectively whereas the LDso of DP5 isolate for one month old
ducklings was determined to be 17 and for adult ducks LDso was
21 cells.
Immunogenic potential of different vaccines prepared
using DP1 and DP5 isolate were tested in ducks by giving two
doses of vaccine. The first dose was given at four weeks of
age and second dose was given 80 days after the first dose.
The birds were challenged with 0.1 ml of culture containing
100 LDso of fully encapsulated virulent form of bacteria at 20
days interval till 80th day and then at 90th and 120th day
after the first dose of vaccine.
A higher percentage of
protection was conferred by oil adjuvant vaccines prepared
using DP1 and DP5 isolate and sonicated antigen with oil
adjuvant prepared using DP1 isolate.
The serum samples were collected from vaccinated birds at
regular intervals of 0 I 7 I 20 I 40 I 60 I 80 I 90 and 120 for
indirect haemagglutination test and the titres obtained were
ranging from 8 to 256. More evaluation and elaborated field
trials are required before advocating bacterin with oil
adjuvant for field use.
| Item type | Current location | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|
Theses
|
KAU Central Library, Thrissur Theses | 636.089 6 JAY/CO (Browse shelf) | Available | 171428 |
MVSc
Two isolates of Pasteurella mul tocida from ducks viz. DP1
and DP5 isolated from ailing/dead ducks from Kuttanad area and
maintained in the virulent form were used separately for the
preparation of different vaccines viz., bacterin, bacterin
with oil adjuvant and sonicated antigen with oil adjuvant.
The biological and biochemical characters of both the
isolates were compared and are in confirmity with the
characters described for the species by earlier workers.
Both the isolates were pathogenic to ducks and mice as
all the inoculated ducks and mice were killed with in 72 h.
The LDso for the DP1 isolate in one month old ducklings and six
month old ducks were determined to be 23 and 32 cells
respectively whereas the LDso of DP5 isolate for one month old
ducklings was determined to be 17 and for adult ducks LDso was
21 cells.
Immunogenic potential of different vaccines prepared
using DP1 and DP5 isolate were tested in ducks by giving two
doses of vaccine. The first dose was given at four weeks of
age and second dose was given 80 days after the first dose.
The birds were challenged with 0.1 ml of culture containing
100 LDso of fully encapsulated virulent form of bacteria at 20
days interval till 80th day and then at 90th and 120th day
after the first dose of vaccine.
A higher percentage of
protection was conferred by oil adjuvant vaccines prepared
using DP1 and DP5 isolate and sonicated antigen with oil
adjuvant prepared using DP1 isolate.
The serum samples were collected from vaccinated birds at
regular intervals of 0 I 7 I 20 I 40 I 60 I 80 I 90 and 120 for
indirect haemagglutination test and the titres obtained were
ranging from 8 to 256. More evaluation and elaborated field
trials are required before advocating bacterin with oil
adjuvant for field use.
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