Standardization of Mass Production Technique for VA Mycorrhiza
By: Saritha V Elizabeth.
Contributor(s): Joseph P J (Guide).
Material type:
BookPublisher: Vellayani Department of Plant Pathology, College of Agriculture 1999DDC classification: 632.3 Online resources: Click here to access online Dissertation note: MSc Abstract: Different methods to standardize the mass inoculum production
techniques of AMF was attempted in the present investigation. Effort to
identify suitable substrate combination indicated that a substrate combination
consisting of basal medium of vermiculite 65 per cent, perlite 20 per cent,
cowdung 5 per cent and soil 10 per cent as components was ideal for maximum
AMF spore production as well as for plant growth.
Mycorrhizal setaria plant was found to be the ideal host for biomass
production while maximum AMF spore count was obtained with stylosanthes,
which possessed only less root volume. A host combination of congosignal
and stylosanthes was found to be the ideal system for achieving maximum AMF
colonization and spore production apart from increased plant growth and
biomass.
Application of different plant growth regulators stimulated plant
growth, biomass production and AMF spore production. Foliar application of
humic acid 2000 ~l r' resulted in maximum increase in spore production and
hence can be recommended for application in routine AMF inoculum
production systems.
Induction of stress to the host plants using different stress inducing
substances stimulated AMF sporulation. Water stress induced on 75 DAP
resulted in maximum spore production. The stress inducers which increased
spore production also include ethrel 25 ul r', NaCI 0.5, 1.0, 1.5 per cent and
ABA 2.0 mg r ' treatments.
Although the plant protection chemicals viz., metalaxyl (1000 mg rl)
and streptomycin (1000 and 2000 mg rl) promoted plant growth, there was
significant reduction in the AMF spore production and hence are unsuitable for
application on hosts intended for AMF inoculum production.
The present study revealed that infective propagules of AMF in the
inoculum could be increased by selecting substrates and host combinations
suitable for inoculum production which could further be boosted up by
applying selective growth promoting substances such as humic acid 2000 III r'
and inducing water stress during the later stages of plant growth.
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MSc
Different methods to standardize the mass inoculum production
techniques of AMF was attempted in the present investigation. Effort to
identify suitable substrate combination indicated that a substrate combination
consisting of basal medium of vermiculite 65 per cent, perlite 20 per cent,
cowdung 5 per cent and soil 10 per cent as components was ideal for maximum
AMF spore production as well as for plant growth.
Mycorrhizal setaria plant was found to be the ideal host for biomass
production while maximum AMF spore count was obtained with stylosanthes,
which possessed only less root volume. A host combination of congosignal
and stylosanthes was found to be the ideal system for achieving maximum AMF
colonization and spore production apart from increased plant growth and
biomass.
Application of different plant growth regulators stimulated plant
growth, biomass production and AMF spore production. Foliar application of
humic acid 2000 ~l r' resulted in maximum increase in spore production and
hence can be recommended for application in routine AMF inoculum
production systems.
Induction of stress to the host plants using different stress inducing
substances stimulated AMF sporulation. Water stress induced on 75 DAP
resulted in maximum spore production. The stress inducers which increased
spore production also include ethrel 25 ul r', NaCI 0.5, 1.0, 1.5 per cent and
ABA 2.0 mg r ' treatments.
Although the plant protection chemicals viz., metalaxyl (1000 mg rl)
and streptomycin (1000 and 2000 mg rl) promoted plant growth, there was
significant reduction in the AMF spore production and hence are unsuitable for
application on hosts intended for AMF inoculum production.
The present study revealed that infective propagules of AMF in the
inoculum could be increased by selecting substrates and host combinations
suitable for inoculum production which could further be boosted up by
applying selective growth promoting substances such as humic acid 2000 III r'
and inducing water stress during the later stages of plant growth.
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