Isoenzyme Variation in Curcuma with Special Reference to Curcuma longa L.
By: Renu Joseph.
Contributor(s): Dr. V. S. Sujatha (Guide).
Material type:
BookPublisher: Vellanikkara Department Of Plantation Crops And Spices,College Of Horticulture 1999DDC classification: 633.8 Online resources: Click here to access online Dissertation note: MSc Abstract: The study on 'Isoenzyme variation in Curcuma with special reference to
C. longa was conducted in the Department of Plantation Crops and Spices and
Biochemistry laboratory at College of Horticulture, Vellanikkara, Thrissur from
October 1997 to June 1999. Eighteen species of genus Curcuma, including
C. longa were included in the study. Thirty three accessions of C. longa were
selected to represent 21 morphotypes of Velayudhan et al. (1994). Six released
varieties were also studied. These were analysed for three enzymes viz. peroxidase,
esterase and GOT using polyacrylamide gel electrophoresis.
The selection of ideal part for electrophoresis for each enzyme was done
based on observations of banding pattern of root, rhizome, sprout and different
maturity stages of leaf. Sampling and analysis was done during three weeks after
sprouting and just before senescence of leaf to standardise the stage of growth of
plant. Mature leaf just before its senescence was selected for peroxidase and GOT
analysis. Tender leaf three weeks after sprouting was found to be ideal for esterase.
lsoenzyrne banding pattern of 39 genotypes of C. longa were studied for
esterase, peroxidase and GOT. The similarity index among the genotypes ranged
from 0.39 to one. The genotypes were classified into 21 groups with similarity index
one among the members. Morphotypes M-I, M-2 and r'A-3 ofVelayudhan et 01
(1994) formed one group with similarity index one. M-7 and M-8 were also grouped
into one. Released varieties under study showed more similarity with first group of
morphotypes.
On grouping the [8 species of Curcuma based on isoenzyme similarity
C zedoaria, C. aeruginosa, C. malabarica, C. comosa and C. cacsia formed one
group, C. amada, C. syivatica, C. aromatica and C. liarita the second group and
C. soloensis, C. brog and C montana the third group. Cultivated type C longo and
other sessile tuberising species C. latifolia and C. raktakanta showed variable
similarity with other species and stood independently. Stoloniferous type C. vamana
showed more similarity with sessile tuberising species than with non-sessile
tuberising species, Non-sessile tuberising species C. aurantiaca and
C. pseudomontana stood individually showing their distinctness from rest of the
species.
| Item type | Current location | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|
Theses
|
KAU Central Library, Thrissur Theses | 633.8 REN/IS (Browse shelf) | Available | 171595 |
MSc
The study on 'Isoenzyme variation in Curcuma with special reference to
C. longa was conducted in the Department of Plantation Crops and Spices and
Biochemistry laboratory at College of Horticulture, Vellanikkara, Thrissur from
October 1997 to June 1999. Eighteen species of genus Curcuma, including
C. longa were included in the study. Thirty three accessions of C. longa were
selected to represent 21 morphotypes of Velayudhan et al. (1994). Six released
varieties were also studied. These were analysed for three enzymes viz. peroxidase,
esterase and GOT using polyacrylamide gel electrophoresis.
The selection of ideal part for electrophoresis for each enzyme was done
based on observations of banding pattern of root, rhizome, sprout and different
maturity stages of leaf. Sampling and analysis was done during three weeks after
sprouting and just before senescence of leaf to standardise the stage of growth of
plant. Mature leaf just before its senescence was selected for peroxidase and GOT
analysis. Tender leaf three weeks after sprouting was found to be ideal for esterase.
lsoenzyrne banding pattern of 39 genotypes of C. longa were studied for
esterase, peroxidase and GOT. The similarity index among the genotypes ranged
from 0.39 to one. The genotypes were classified into 21 groups with similarity index
one among the members. Morphotypes M-I, M-2 and r'A-3 ofVelayudhan et 01
(1994) formed one group with similarity index one. M-7 and M-8 were also grouped
into one. Released varieties under study showed more similarity with first group of
morphotypes.
On grouping the [8 species of Curcuma based on isoenzyme similarity
C zedoaria, C. aeruginosa, C. malabarica, C. comosa and C. cacsia formed one
group, C. amada, C. syivatica, C. aromatica and C. liarita the second group and
C. soloensis, C. brog and C montana the third group. Cultivated type C longo and
other sessile tuberising species C. latifolia and C. raktakanta showed variable
similarity with other species and stood independently. Stoloniferous type C. vamana
showed more similarity with sessile tuberising species than with non-sessile
tuberising species, Non-sessile tuberising species C. aurantiaca and
C. pseudomontana stood individually showing their distinctness from rest of the
species.
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