Improvement of in Vitro somatic embryogenesis in Cashew (Anacardium occidentale L.)
By: Rekha S.
Contributor(s): Sulekha G R (Guide).
Material type:
BookPublisher: Vellayani Department of Horticulture, College of Agriculture 1999DDC classification: 635 Online resources: Click here to access online Dissertation note: MSc Abstract: Studies were conducted for improving techniques for in vitro
somatic embryogenesis in cashew during 1996-1999 in the Plant Tissue
Culture Laboratory of the Department of Horticulture, College of
Agriculture, Vellayani. Attempts were made to standardise the various
stages of somatic embryogenesis, namely, induction, initiation, maturation
and germination using nucellus and embryo mass as explants.
Out of the two explants tried, nucellus responded better than
embryo mass in initiating embryogenic callus/somatic embryoids.
Induction of somatic embryogenesis from nucellus was found to occur
at its maximum when cultured in darkness on MS basal medium having half
strength major salts, supplemented with 2,4-D 1.0 mg/l, BA 1.0 mg/l, NAA
1.0 mg/l, sucrose 30.0 g/l, Activated charcoal 0.5 g/l and agar 6.0 g/l.
The best treatment identified for the induction of embryogenic
callus / somatic embryoids from embryo mass was MS basal medium
supplemented with 2,4-D 4.0 mg/l, NAA 4.0 mg/l, kinetin 4.0 mg/l,
adenine sulphate 40.0 mg/l, yeast extract 200.0 mg/l, PVP 250.0 mg/l,
sucrose 30.0 g/l and agar 5.5 g/l, in dark culture condition at regulated
temperature (26 ± 2°C).
2
Initiation of somatic embryoids from nucellus as well as embryo
mass occurred at its best in darkness on MS basal medium supplemented
with NAA 0.5 mg/l, kinetin 2.0 mg/l, adenine sulphate 40.0 mg/l, PVP
250.0 mg/l, yeast extract 200.0 mg/l, sucrose 30.0 g/l and agar 5.5 g/l.
. Among the treatments tried for inducing proper maturation of the
somatic embryoids, the maximum survival of embryoids was recorded on
a combination of basal media with Bs major salts and MS minor salts
supplemented with ABA 1.0 mg/l, coconut water 200.0 mlll, casein
hydrolysate 100.0 mg/l, PVP 10.0 g/l, sucrose 40.'0 g/l and agar 5.0 g/l.
Culture conditions did not influence the maturation process of
the somatic embryoids.
Maturation process was not found to be essential in inducing
normal germination of the somatic embryoids. The cultures showed good
response when subcultured from initiation media to germination media
without a maturation phase.
Germination of somatic embryoids occurred only in the presence
of light, on a combination of basal media with Bs major salts and MS
minor salts supplemented with BA 1.0 mg/l, PVP 10.0 g/l, coconut water
200.0 mlll, sodium chloride 0.1 per cent, cobalt chloride 10.0 g/l, sucrose
50.0 g/l and agar 6.0 g/l.
| Item type | Current location | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|
Theses
|
KAU Central Library, Thrissur Theses | 635 REK/IM (Browse shelf) | Available | 171602 |
MSc
Studies were conducted for improving techniques for in vitro
somatic embryogenesis in cashew during 1996-1999 in the Plant Tissue
Culture Laboratory of the Department of Horticulture, College of
Agriculture, Vellayani. Attempts were made to standardise the various
stages of somatic embryogenesis, namely, induction, initiation, maturation
and germination using nucellus and embryo mass as explants.
Out of the two explants tried, nucellus responded better than
embryo mass in initiating embryogenic callus/somatic embryoids.
Induction of somatic embryogenesis from nucellus was found to occur
at its maximum when cultured in darkness on MS basal medium having half
strength major salts, supplemented with 2,4-D 1.0 mg/l, BA 1.0 mg/l, NAA
1.0 mg/l, sucrose 30.0 g/l, Activated charcoal 0.5 g/l and agar 6.0 g/l.
The best treatment identified for the induction of embryogenic
callus / somatic embryoids from embryo mass was MS basal medium
supplemented with 2,4-D 4.0 mg/l, NAA 4.0 mg/l, kinetin 4.0 mg/l,
adenine sulphate 40.0 mg/l, yeast extract 200.0 mg/l, PVP 250.0 mg/l,
sucrose 30.0 g/l and agar 5.5 g/l, in dark culture condition at regulated
temperature (26 ± 2°C).
2
Initiation of somatic embryoids from nucellus as well as embryo
mass occurred at its best in darkness on MS basal medium supplemented
with NAA 0.5 mg/l, kinetin 2.0 mg/l, adenine sulphate 40.0 mg/l, PVP
250.0 mg/l, yeast extract 200.0 mg/l, sucrose 30.0 g/l and agar 5.5 g/l.
. Among the treatments tried for inducing proper maturation of the
somatic embryoids, the maximum survival of embryoids was recorded on
a combination of basal media with Bs major salts and MS minor salts
supplemented with ABA 1.0 mg/l, coconut water 200.0 mlll, casein
hydrolysate 100.0 mg/l, PVP 10.0 g/l, sucrose 40.'0 g/l and agar 5.0 g/l.
Culture conditions did not influence the maturation process of
the somatic embryoids.
Maturation process was not found to be essential in inducing
normal germination of the somatic embryoids. The cultures showed good
response when subcultured from initiation media to germination media
without a maturation phase.
Germination of somatic embryoids occurred only in the presence
of light, on a combination of basal media with Bs major salts and MS
minor salts supplemented with BA 1.0 mg/l, PVP 10.0 g/l, coconut water
200.0 mlll, sodium chloride 0.1 per cent, cobalt chloride 10.0 g/l, sucrose
50.0 g/l and agar 6.0 g/l.
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