Enzyme Immuno Assay Based Seromonitoring and Detection of Canine Rabiesb Antigen
By: Biju K G.
Contributor(s): Krishnan Nair G (Guide).
Material type:
BookPublisher: Mannuthy Department of Microbiology, College of Veterinary and Animal Sciences 1999DDC classification: 636.089 6 Online resources: Click here to access online Dissertation note: MVSc Abstract: In the present study an attempt was made to measure the serum
neutralizing antibodies against rabies virus in the sera of both vaccinated and
unvaccinated dogs, employing ELISA. A long with this special emphasis was
given to compare the results of diagnostic techniques used for yaJ.i.es namely
Seller's staining and IP test.
Four hundred and thirty-four sera samples from dogs of different
age groups were screened. Enzyme immuno assay of sera of 83 unvaccinated
pups revealed that under the age group of 0-3 months 59.1 percent antibody titre
of 1: 50 or above indicating the presence of maternal antibodies.
Out of 116 sera samples from primarly vaccinated group only
eight had antibody titre below 1:50. Among the booster vaccinated 94 sera
samples tested only four samples showed antibody titre below 1:50 ..
The assay revealed that during diseases the immune systems of
the dogs will be suppressed. It was found that the age of first vaccination could
be decided as third month. The present study revealed that primary vaccination
might not produces higher antibody titres in dogs. The study showed advantages
of booster vaccination over primary vaccination.
All the rabies suspected cases were subjected as two diagnostic
tests namely Seller's staining and IPT. Out of 20 rabies suspected cases, there
were detected by demonstration of Negri bodies and four were by IPT.
The present study showed plate ELISA is a sensitive method for
titration of rabies virus neutralizing antibodies and IPT can be successfully used
for the detection of rabies antigen in impression smears taken from
hippocampus.
| Item type | Current location | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|
Theses
|
KAU Central Library, Thrissur Theses | 636.089 6 BIJ/EN (Browse shelf) | Available | 171631 |
MVSc
In the present study an attempt was made to measure the serum
neutralizing antibodies against rabies virus in the sera of both vaccinated and
unvaccinated dogs, employing ELISA. A long with this special emphasis was
given to compare the results of diagnostic techniques used for yaJ.i.es namely
Seller's staining and IP test.
Four hundred and thirty-four sera samples from dogs of different
age groups were screened. Enzyme immuno assay of sera of 83 unvaccinated
pups revealed that under the age group of 0-3 months 59.1 percent antibody titre
of 1: 50 or above indicating the presence of maternal antibodies.
Out of 116 sera samples from primarly vaccinated group only
eight had antibody titre below 1:50. Among the booster vaccinated 94 sera
samples tested only four samples showed antibody titre below 1:50 ..
The assay revealed that during diseases the immune systems of
the dogs will be suppressed. It was found that the age of first vaccination could
be decided as third month. The present study revealed that primary vaccination
might not produces higher antibody titres in dogs. The study showed advantages
of booster vaccination over primary vaccination.
All the rabies suspected cases were subjected as two diagnostic
tests namely Seller's staining and IPT. Out of 20 rabies suspected cases, there
were detected by demonstration of Negri bodies and four were by IPT.
The present study showed plate ELISA is a sensitive method for
titration of rabies virus neutralizing antibodies and IPT can be successfully used
for the detection of rabies antigen in impression smears taken from
hippocampus.
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