Micropropagation of teak (Tectona Grandis Linn.) through in vitro techniques
By: Sandeep Sharma.
Contributor(s): Vijayakumar N K (Guide).
Material type:
BookPublisher: Vellanikkara Department of Tree Physiology and Breeding, College of Forestry, 2000DDC classification: 634.9 Online resources: Click here to access online Dissertation note: MSc Abstract: The study under the title 'Micropropagation of teak (Tectona grandis
Linn.) through in vitro techniques' was carried out at College of Forestry
Vellanikkara during year 1998-2000. The objective of the programme is to
standardize the method of clonal propagation of teak through teak tissue culture
technique. The explants used were from one to two year old seedlings
Culture contamination mainly due to fungus was prominent in the
ramy season. Prophylactic spraying of a mixture of fungicide (Bavistin and
Indofil M-45 (0.1% each) on source material followed by fungicide dip to
explants in similar solution for 30 min and surface sterilization treatment in 0.15
per cent mercuric chloride for 15 min controlled the contamination. Phenol
exudation was contained effectively by supplementation of citric acid and
ascorbic acid (150 mgl' each), to the media. It was also in low quantity when
explants of smallest size (1 cm long below the node) were used.
Murashige and Skoog (MS) medium was found to be better than 12
MS and WPM. Individual supplementation of BA to MS media was found
effective than other cytokinins. However, MS media supplemented with Kinetin
and !AA 0.5mgrl each was found to be the best media for shoot proliferation. A
maximum of2.20 and 2.11 number of shoot shoots per explant could be induced
in MS + 0.5 mg l' BA and MS + 1.0 mg l' BA + 0.1 mg rl!AA + 1.0 mg I-I 2ip
respectively. Addition of growth supplements like coconut water, activated
charcoal, adenine sulphate, and casein hydrolysate did not have any favourable
effect on growth and establishment.
Maximum in vitro rooting (56.25%) was obtained on Yz MS + 0.4
mg r' NAA + 4.0 mg rl !AA +0.25 per cent AC with pulse treatment (dip to
excised shoot base in 1000 mg r' !AA solution for 2 min) and after transferring
to auxin free media after 7 days. Under ex vitro rooting trials the maximum
percentage of rooting (87.50) with highest root length (3.0 cm) was obtained on
vermiculite. Sand and vemiciilite were found to be the best media for hardening
of in vitro raised plantlets. The hardened plants were transplanted to polybags
containing ordinary potting mixture and treated like a normal conditioned
seedling. Hardened seedlings were out planted in the field.
The technique developed for the micropropagation of teak during
present investigation can be used for large scale clonal multiplication of the
species.
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Theses
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KAU Central Library, Thrissur Theses | 634.9 SAN/MI (Browse shelf) | Available | 171698 |
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MSc
The study under the title 'Micropropagation of teak (Tectona grandis
Linn.) through in vitro techniques' was carried out at College of Forestry
Vellanikkara during year 1998-2000. The objective of the programme is to
standardize the method of clonal propagation of teak through teak tissue culture
technique. The explants used were from one to two year old seedlings
Culture contamination mainly due to fungus was prominent in the
ramy season. Prophylactic spraying of a mixture of fungicide (Bavistin and
Indofil M-45 (0.1% each) on source material followed by fungicide dip to
explants in similar solution for 30 min and surface sterilization treatment in 0.15
per cent mercuric chloride for 15 min controlled the contamination. Phenol
exudation was contained effectively by supplementation of citric acid and
ascorbic acid (150 mgl' each), to the media. It was also in low quantity when
explants of smallest size (1 cm long below the node) were used.
Murashige and Skoog (MS) medium was found to be better than 12
MS and WPM. Individual supplementation of BA to MS media was found
effective than other cytokinins. However, MS media supplemented with Kinetin
and !AA 0.5mgrl each was found to be the best media for shoot proliferation. A
maximum of2.20 and 2.11 number of shoot shoots per explant could be induced
in MS + 0.5 mg l' BA and MS + 1.0 mg l' BA + 0.1 mg rl!AA + 1.0 mg I-I 2ip
respectively. Addition of growth supplements like coconut water, activated
charcoal, adenine sulphate, and casein hydrolysate did not have any favourable
effect on growth and establishment.
Maximum in vitro rooting (56.25%) was obtained on Yz MS + 0.4
mg r' NAA + 4.0 mg rl !AA +0.25 per cent AC with pulse treatment (dip to
excised shoot base in 1000 mg r' !AA solution for 2 min) and after transferring
to auxin free media after 7 days. Under ex vitro rooting trials the maximum
percentage of rooting (87.50) with highest root length (3.0 cm) was obtained on
vermiculite. Sand and vemiciilite were found to be the best media for hardening
of in vitro raised plantlets. The hardened plants were transplanted to polybags
containing ordinary potting mixture and treated like a normal conditioned
seedling. Hardened seedlings were out planted in the field.
The technique developed for the micropropagation of teak during
present investigation can be used for large scale clonal multiplication of the
species.
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