Isolation And Characterization Of Chlamyda Psittaci With Emphasis On Protein Profile
By: Binu K Mani.
Contributor(s): Mini M (Guide).
Material type:
BookPublisher: Mannuthy Department of Microbiology, College of Veterinary and Animal Sciences 2001DDC classification: 636.089 6 Online resources: Click here to access online Dissertation note: MVSc Abstract: For isolation of Chlamydia psittaci 46 clinical
cases associated with abortions in livestock from
livestock farms and Veterinary hospitals in and around
Thrissur were screened. Impression smear staining of
the clinical materials USin9 Giemsa, Modified Ziehl
Neelsen and Gimenez revealed two positive cases. Both
were from aborted foetuses, one from bovine (M-121)
and the other from caprine (M-430).
Chick embryo inoculation by YS route was used for
isolation of the organism. The organisms could be
isolated only
from the
two
cases
positive by
preliminary screening, as confirmed by staining of YS
impression smears.
The mortality rate in CE was more in case of
M-430 compared to the other two isolates (M-121 and
reference isolate, P-156). All the three isolates
produced patchy haemorrhagic lesions in YS and skin of
embryoS. An overall isolation percentage of 4.3 was
obtained in this study.
All the three isolates were resistant to sodium
sulphadiazine.
Pathogenicity studies indicated that, all the
three isolates were of moderate virulence for mice and
guinea pigs.
Based on AGPT, the local isolates were confirmed
as Chlamydia psittaci.
All the three isolates caused rounding and
swelling of fibroblast cells and syncytia formation at
24h PI in Mc Coy cell line. By 48 to 72 h PI, these
effects became more prominent with detachment of cells
from adjacent cells and from the glass surface.
Purified EBs were prepared from infected Mc Coy
cell line by Urografin gradient centrifugation. After
confirming the presence of sufficient amount of
protein by Biuret method, they were used for SDS-PAGE.
A total of 12 polypeptide bands were obtained for both
the bovine isolates while the caprine isolate gave 10
bands. Molecular weights of 148 kDa and 135 kDa were
present only in P-156 and 152 kDa and 137 kDa were
unique for M-121. Bands at 152 kDa, 148 kDa, 137 kDa,
135 kDa, 32 kDa, 18 kDa, 15 kDa and 7 kDa were lacking
in M-430. This isolate was having unique bands of
155 kDa, 19 kDa, 12.2 kDa and 6.4 kDa.
143
| Item type | Current location | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|
Theses
|
KAU Central Library, Thrissur Theses | 636.089 6 BIN/IS (Browse shelf) | Available | 171766 |
MVSc
For isolation of Chlamydia psittaci 46 clinical
cases associated with abortions in livestock from
livestock farms and Veterinary hospitals in and around
Thrissur were screened. Impression smear staining of
the clinical materials USin9 Giemsa, Modified Ziehl
Neelsen and Gimenez revealed two positive cases. Both
were from aborted foetuses, one from bovine (M-121)
and the other from caprine (M-430).
Chick embryo inoculation by YS route was used for
isolation of the organism. The organisms could be
isolated only
from the
two
cases
positive by
preliminary screening, as confirmed by staining of YS
impression smears.
The mortality rate in CE was more in case of
M-430 compared to the other two isolates (M-121 and
reference isolate, P-156). All the three isolates
produced patchy haemorrhagic lesions in YS and skin of
embryoS. An overall isolation percentage of 4.3 was
obtained in this study.
All the three isolates were resistant to sodium
sulphadiazine.
Pathogenicity studies indicated that, all the
three isolates were of moderate virulence for mice and
guinea pigs.
Based on AGPT, the local isolates were confirmed
as Chlamydia psittaci.
All the three isolates caused rounding and
swelling of fibroblast cells and syncytia formation at
24h PI in Mc Coy cell line. By 48 to 72 h PI, these
effects became more prominent with detachment of cells
from adjacent cells and from the glass surface.
Purified EBs were prepared from infected Mc Coy
cell line by Urografin gradient centrifugation. After
confirming the presence of sufficient amount of
protein by Biuret method, they were used for SDS-PAGE.
A total of 12 polypeptide bands were obtained for both
the bovine isolates while the caprine isolate gave 10
bands. Molecular weights of 148 kDa and 135 kDa were
present only in P-156 and 152 kDa and 137 kDa were
unique for M-121. Bands at 152 kDa, 148 kDa, 137 kDa,
135 kDa, 32 kDa, 18 kDa, 15 kDa and 7 kDa were lacking
in M-430. This isolate was having unique bands of
155 kDa, 19 kDa, 12.2 kDa and 6.4 kDa.
143
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