Seroprevalence Of Hydropericardium Syndrome In Broiler Chickens
By: Binu TV.
Contributor(s): Koshy John (Guide).
Material type:
BookPublisher: Mannuthy Department of Microbiology, College of Veterinary and Animal Sciences 2000DDC classification: 636.089 6 Online resources: Click here to access online Dissertation note: MVSc Abstract: In
the
present
study,
seroprevalence
of
hydropericardium syndrome (HPS) in broiler chickens in
Kerala was
evaluated.
The
results
of agar
gel
precipitation test (AGPT), counter imrnunoelectrophoresis
(eIE) and indirect enzyme linked imrnunosorbent assay
(ELISA) were compared in detecting HPS antibodies.
A total of 350 sera samples were collected from
different parts of Kerala. In the first phase, 50 sera
samples were screened by AGPT, eIE and indirect ELISA.
Three samples were found positive by AGPT and eIE.
Seven samples were found to be posi ti ve by indirect
ELISA. Based on the comparison, another 300 samples
were screened using indirect ELISA and 17 samples were
found to be positive.
A total of 24 samples were found positive for HPS
antibodies out of 350 samples screened by indirect
ELISA.
The seroprevalence of 6.86 percentage was
evaluated in broiler chickens in Kerala using indirect
ELISA.
The syndrome was experimentally reproduced in
three-week-old broiler
subcutaneous
inoculation
viz. ,
chicks.
intramuscular,
Various
routes
of
and
intraperitoneal routes were tried. The lesions were
more pronounced when the birds were inoculated by
subcutaneous route. Chick mortality was high on the
fourth day.
Electron
microscopy
of
the
infected
liver
homogenate revealed hexagonal virus particles of about
75 nm in size. These are suggestive of adenovirus.
Histopathology
of
necropsied
birds
showed
degeneration and necrosis of myocardial fibres,
necrosis of hepatocytes and focal areas of coagulation
in liver. Basophilic intranuclear inclusion bodies were
demonstrated in hepatocytes. Vacuolar degeneration of
kidney tubules, lymphoid depletion of bursa, vascular
sclerosis of spleen and congestion and oedema of lungs
were also seen.
| Item type | Current location | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|
Theses
|
KAU Central Library, Thrissur Theses | 636.089 6 BIN/SE (Browse shelf) | Available | 171767 |
MVSc
In
the
present
study,
seroprevalence
of
hydropericardium syndrome (HPS) in broiler chickens in
Kerala was
evaluated.
The
results
of agar
gel
precipitation test (AGPT), counter imrnunoelectrophoresis
(eIE) and indirect enzyme linked imrnunosorbent assay
(ELISA) were compared in detecting HPS antibodies.
A total of 350 sera samples were collected from
different parts of Kerala. In the first phase, 50 sera
samples were screened by AGPT, eIE and indirect ELISA.
Three samples were found positive by AGPT and eIE.
Seven samples were found to be posi ti ve by indirect
ELISA. Based on the comparison, another 300 samples
were screened using indirect ELISA and 17 samples were
found to be positive.
A total of 24 samples were found positive for HPS
antibodies out of 350 samples screened by indirect
ELISA.
The seroprevalence of 6.86 percentage was
evaluated in broiler chickens in Kerala using indirect
ELISA.
The syndrome was experimentally reproduced in
three-week-old broiler
subcutaneous
inoculation
viz. ,
chicks.
intramuscular,
Various
routes
of
and
intraperitoneal routes were tried. The lesions were
more pronounced when the birds were inoculated by
subcutaneous route. Chick mortality was high on the
fourth day.
Electron
microscopy
of
the
infected
liver
homogenate revealed hexagonal virus particles of about
75 nm in size. These are suggestive of adenovirus.
Histopathology
of
necropsied
birds
showed
degeneration and necrosis of myocardial fibres,
necrosis of hepatocytes and focal areas of coagulation
in liver. Basophilic intranuclear inclusion bodies were
demonstrated in hepatocytes. Vacuolar degeneration of
kidney tubules, lymphoid depletion of bursa, vascular
sclerosis of spleen and congestion and oedema of lungs
were also seen.
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