Nucleic Acid And Protein Profile Of Pasteurella Multocida Of Avian Origin
By: Rajalakshmi Radabai S.
Contributor(s): Krishnan Nair G (Guide).
Material type:
BookPublisher: Mannuthy Department of Microbiology, College of Veterinary and Animal Sciences 2001DDC classification: 636.089 6 Online resources: Click here to access online Dissertation note: MVSc Abstract: Four avian field isolates (one from quail and three
from ducks) suggestive of Pasteurella sp. were compared with the
avian Pasteurella multocida reference strain (LKO) for their
biochemical properties, drug resistance patterns, whole cell and outer
membrane protein profiles and restriction enzyme digestion pattern
of chromosomal DNA.
Biochemical characterisation revealed two biotypes
among the four field isolates; namely P. multocida subsp. septica
(quail isolate) and P. multocida subsp. multocida (duck isolates).
The study of antibiogram using 14 chemotherapeutic
agents revealed the presence of multiple drug resistance in all the
four field isolates and reference strain.
Whole cell protein profiles of the four field isolates and
reference strain revealed 7 to 11 bands of molecular weights
ranging from 197.4 kDa to 2.5 kDa. Polypeptide bands in the
molecular weight range of 36 to 38 kDa (which were attributable to
the protein-H), 29 to 32 kDa and 10 to 11 kDa were common to all
the isolates of P. multocida.
The major outer membrane proteins (OMPs) in the
molecular weight range of 32 to 33 kDa and 27 kDa region were
common to all the four field isolates and reference strain.
II
Unique protein bands in each of the isolate were
indicative of variant forms of the same organism.
Restriction enzyme analysis of chromosomal DNA
yielded different restriction fragments in each of the isolates which
were not easily distinguishable.
Hence, of the tests used, phenotypic characters, biotype,
antibiogram and protein profile were found to be more effective in
differentiating the isolates rather than restriction enzyme digestion
patterns of genomic DNA.
| Item type | Current location | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|
Theses
|
KAU Central Library, Thrissur Theses | 636.089 6 RAJ/Nu (Browse shelf) | Available | 171780 |
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MVSc
Four avian field isolates (one from quail and three
from ducks) suggestive of Pasteurella sp. were compared with the
avian Pasteurella multocida reference strain (LKO) for their
biochemical properties, drug resistance patterns, whole cell and outer
membrane protein profiles and restriction enzyme digestion pattern
of chromosomal DNA.
Biochemical characterisation revealed two biotypes
among the four field isolates; namely P. multocida subsp. septica
(quail isolate) and P. multocida subsp. multocida (duck isolates).
The study of antibiogram using 14 chemotherapeutic
agents revealed the presence of multiple drug resistance in all the
four field isolates and reference strain.
Whole cell protein profiles of the four field isolates and
reference strain revealed 7 to 11 bands of molecular weights
ranging from 197.4 kDa to 2.5 kDa. Polypeptide bands in the
molecular weight range of 36 to 38 kDa (which were attributable to
the protein-H), 29 to 32 kDa and 10 to 11 kDa were common to all
the isolates of P. multocida.
The major outer membrane proteins (OMPs) in the
molecular weight range of 32 to 33 kDa and 27 kDa region were
common to all the four field isolates and reference strain.
II
Unique protein bands in each of the isolate were
indicative of variant forms of the same organism.
Restriction enzyme analysis of chromosomal DNA
yielded different restriction fragments in each of the isolates which
were not easily distinguishable.
Hence, of the tests used, phenotypic characters, biotype,
antibiogram and protein profile were found to be more effective in
differentiating the isolates rather than restriction enzyme digestion
patterns of genomic DNA.
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