PhD

A study was undertaken to find out correlation's if any. between somatic
cell count and chemical composition of cross-bred cow milk; the influence of age
of the cow. parity. stage of lactation and season on somatic cell count of milk;
evaluate the effectiveness of California Mastitis Test (CMT) in detecting subclinical
mastitis; find out whether chemical preservatives, formalin and potassium
dichromate will be effective in preserving cells in milk; assess the difference in
concentration of somatic cells bet.ween fractions of milk collected during milking
and to evaluate the effects of mopping of udder and teat. dipping in controlling high
somatic cell count in milk
A modified procedure of Membrane Filter- Deoxyribo Nucleic Acid (MF-
DNA) method was developed and standardised with Direct Microscopic Somatic
Cell Count as well as using pure DNA for estimation of somatic cell count. This
method was precise and suitable for field use. California Mastitis Test (CMT)
scores has good correlation with somatic cell count and is useful for selecting milk
for processing into various products based on somatic cell count. On analysis of six
hundred individual morning milk samples it was found that lactose per cent is
negatively correlated with somatic cell count Total Solids (TS) and Solids-Not-Fat
(SNF) per cent of milk had a negative correlation with somatic cell count in samples
with fat per cent between 35 and 45 or SNF per cent between 85 and 8.99.
Above groups of samples constituted a major share of total samples collected.

Chloride content of milk had no significant correlation with somatic cell count. Use
of low lactose level in milk as an indicator for high somatic cell count is
recommended, Somatic cell count had a negative correlation with milk yield of
cows, A study on pooled milk samples from milk co-operatives revealed that
TS per cent was significantly lower during cold and wet season (June, July and
August) than warm and dry season (December and January),
There was no significant difference among foremilk" mid-stream milk and
strippings in somatic cell count and CMT may be done on any fraction of milk,
Study revealed that. formalin (OA per cent) may be used as a preservative for milk
samples meant for Direct Microscopic Somatic Cell Count (DMSCC) while
potassium dichromate is not fit for this purpose, None of these preservatives were
not suitable for milk analysis by MF"DNA method of somatic cell count. The
experiment proved that teat dipping was an effective practice in comparison with
mopping of udder prior to milking in controlling high somatic cell count in milk,