Molecular characterization of piper species usiing RAPD technique
By: Murugan C.
Contributor(s): Sujatha V S (Guide).
Material type:
BookPublisher: Vellanikkara Department of Plantation Crops and Spices, College of Horticulture 2002DDC classification: 633.8 Online resources: Click here to access online Dissertation note: MSc Abstract: The study on "Molecular characterization of Piper species using RAPD
techniques" was conducted in the Department of Plantation Crops and Spices and
CPBMB, College of Horticulture, Vellanikkara, Thrissur from December, 1999 to
August, 2001. Material included 13 species/accessions of the genus Piper including
Piper nigrutn Linn. These were analysed with 20 selected oligomer decamer primers
using PCR technique and agarose gel electrophoresis.
The selection of ideal DNA isolation technique was done based on
observations of purity of bands, quality of the DNA bands and quantity of DNA
recovered. The protocol of Edwards et al. (1991) was selected for DNA isolation
with additional step of chloroform-isoamyl alcohol treatment.
Fifty decamer primers were screened using DNA. of P. nigrum and 20
were selected based on the number of bands produced, reproducibility of the bands
and distinctness of the RAPD profile.
RAPD profile of the 13 species of Piper were compared for genetic
similarity index (using Jaccard's coefficient), with all 20 selected primers
separately. Pooled similarity of the twenty primers put together was found out for 9
species which gave amplification for all the 20 selected primers. The results of
pooled analysis was subjected to cluster analysis (SAl-IN) and phenetic dcndrogram
was constructed employing UPGMA.
Four clusters were obtained, comprising of two accessions of P. nigrum
In the first, two accessions of P. longum in the second, P. colubrinutn and
P. attenuatum forming the third cluster and P. chaba, P. belle and P. arboreum
forming the fourth.
| Item type | Current location | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|
Theses
|
KAU Central Library, Thrissur Theses | 633.8 MUR/MO (Browse shelf) | Available | 171920 |
MSc
The study on "Molecular characterization of Piper species using RAPD
techniques" was conducted in the Department of Plantation Crops and Spices and
CPBMB, College of Horticulture, Vellanikkara, Thrissur from December, 1999 to
August, 2001. Material included 13 species/accessions of the genus Piper including
Piper nigrutn Linn. These were analysed with 20 selected oligomer decamer primers
using PCR technique and agarose gel electrophoresis.
The selection of ideal DNA isolation technique was done based on
observations of purity of bands, quality of the DNA bands and quantity of DNA
recovered. The protocol of Edwards et al. (1991) was selected for DNA isolation
with additional step of chloroform-isoamyl alcohol treatment.
Fifty decamer primers were screened using DNA. of P. nigrum and 20
were selected based on the number of bands produced, reproducibility of the bands
and distinctness of the RAPD profile.
RAPD profile of the 13 species of Piper were compared for genetic
similarity index (using Jaccard's coefficient), with all 20 selected primers
separately. Pooled similarity of the twenty primers put together was found out for 9
species which gave amplification for all the 20 selected primers. The results of
pooled analysis was subjected to cluster analysis (SAl-IN) and phenetic dcndrogram
was constructed employing UPGMA.
Four clusters were obtained, comprising of two accessions of P. nigrum
In the first, two accessions of P. longum in the second, P. colubrinutn and
P. attenuatum forming the third cluster and P. chaba, P. belle and P. arboreum
forming the fourth.
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