Biomodulation of Non-Specific Immune Response in the Tiger Shrimp Penaeus MonodonFabricus With spirulina Iccorporated Diet
By: Jadhav visal Shripatro.
Contributor(s): Devika Pillai (Guide).
Material type:
BookPublisher: Panangad Department of Aquaculture, College of Fisheries 2002DDC classification: 639.2 Online resources: Click here to access online Dissertation note: MFSc Abstract: Black tiger shrimp; Penaeus monodon is an important species
cultured all over the world. However, disease outbreaks have caused
senous economic losses in several countries. For successful farming of
shrimps disease prevention is a prime necessity The present study was
designed to assess the immunostimulating potential of Spirulina and to see
its effect on growth as well as colour of P. monodon juveniles.
In this study, juveniles of P. monodon were used for evaluating the
effect of different levels of Spirulina on growth, food conversion ratio, food
conversion efficiency, survival as well as prophylactic potency of Spirulina
evaluated by proPhenoloxidase activity, antibacterial assay, haemagglutinin
assay, phagocytosis and challenge study Effect of Spirulina on colour of
shrimps was also studied. Five experimental diets designed as T I to T 5 were
prepared by using clam meat, Spirulina, rice bran, cod liver oil and vitamin-
mineral mix. The total protein content of all diets were kept near to 40%
and Spirulina was incorporated at 0%, 10%, 20%, 30% and 40% in diets by
replacing clam meat. Completely randomized design with five treatments
each having four replications was used for analyzing the results. In each
tank ten numbers of Penaeus monodon juveniles were kept. Feeding was
done for a period of 35 days.
The test animals fed with diet containing Spirulina up to 30%
incorporation showed better growth, food conversion ratio and food
conversion efficiency. But survival during experiment was not affected by
the addition of Spirulina. Spirulina incorporated treatments showed higher
phenoloxidase activity than control. Antibacterial activity was also
observed in the treatments incorporated with Spirulina, while in control no
antibacterial activity was observed. Result of agglutinin activity was not
99
clear as control also showed agglutination in undiluted haemolymph sample
and partial agglutination in 1:2 dilution of haemolymph. Spirulina
incorporated treatments showed agglutination up to 1:4 dilution of
haemolymph. But in 1:4 dilution only partial agglutination was observed.
Increased adherence of haemocyte to yeast cells was observed indicating
higher phagocytic activity in the treatment incorporated with Spirulina,
while adherence of haemocyte was rarely observed in control. On challenge
with Vibrio parahaemolyticus, shrimps treated with Spirulina incorporated
diet showed delayed mortality as compared to control.
Spirulina treated shrimps showed darker colouration than control.
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Theses
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KAU Central Library, Thrissur Theses | 639.2 JAD/BI (Browse shelf) | Available | 172069 |
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MFSc
Black tiger shrimp; Penaeus monodon is an important species
cultured all over the world. However, disease outbreaks have caused
senous economic losses in several countries. For successful farming of
shrimps disease prevention is a prime necessity The present study was
designed to assess the immunostimulating potential of Spirulina and to see
its effect on growth as well as colour of P. monodon juveniles.
In this study, juveniles of P. monodon were used for evaluating the
effect of different levels of Spirulina on growth, food conversion ratio, food
conversion efficiency, survival as well as prophylactic potency of Spirulina
evaluated by proPhenoloxidase activity, antibacterial assay, haemagglutinin
assay, phagocytosis and challenge study Effect of Spirulina on colour of
shrimps was also studied. Five experimental diets designed as T I to T 5 were
prepared by using clam meat, Spirulina, rice bran, cod liver oil and vitamin-
mineral mix. The total protein content of all diets were kept near to 40%
and Spirulina was incorporated at 0%, 10%, 20%, 30% and 40% in diets by
replacing clam meat. Completely randomized design with five treatments
each having four replications was used for analyzing the results. In each
tank ten numbers of Penaeus monodon juveniles were kept. Feeding was
done for a period of 35 days.
The test animals fed with diet containing Spirulina up to 30%
incorporation showed better growth, food conversion ratio and food
conversion efficiency. But survival during experiment was not affected by
the addition of Spirulina. Spirulina incorporated treatments showed higher
phenoloxidase activity than control. Antibacterial activity was also
observed in the treatments incorporated with Spirulina, while in control no
antibacterial activity was observed. Result of agglutinin activity was not
99
clear as control also showed agglutination in undiluted haemolymph sample
and partial agglutination in 1:2 dilution of haemolymph. Spirulina
incorporated treatments showed agglutination up to 1:4 dilution of
haemolymph. But in 1:4 dilution only partial agglutination was observed.
Increased adherence of haemocyte to yeast cells was observed indicating
higher phagocytic activity in the treatment incorporated with Spirulina,
while adherence of haemocyte was rarely observed in control. On challenge
with Vibrio parahaemolyticus, shrimps treated with Spirulina incorporated
diet showed delayed mortality as compared to control.
Spirulina treated shrimps showed darker colouration than control.
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