MVSc

A study was conducted in the Department of Clinical Medicine, College
of Veterinary and Animal Sciences, Mannuthy, for a period of three semesters
during the year 2002 and 2003, to assess the efficacy of oral administration oftri-
sodium citrate as therapeutic agent in subclinical mastitis of cattle and as a
supportive treatment along with antibiotic in clinical mastitis of cattle and to
evaluate the levels of pH, citric acid, calcium, lactose and chloride in milk of
cattle affected with mastitis.
Twenty cases of subclinical mastitis and fifteen cases of clinical mastitis
were utilized for the study. Subclinical mastitis cases were divided in to three
groups and clinical mastitis cases divided into two groups based on the
therapeutic trial adopted.
Gram positive cocci were isolated from cases in which the milk appeared
white, cream, slight yellow or yellow coloured with fine clots or flakes. Gram
negative coccobacilli were isolated from cases in which the milk appeared straw
or yellow coloured watery milk with clots or without clots. Gram positive cocci
were isolated from majority of the subclinical and clinical mastitis cases than the
gram negative coccobacilli.
Gram positive cocci were, highly sensitive to enrofloxacin, cephotaxime
and ciprofloxacin. Gram negative coccobacilli were highly sensitive to
chloramphenicol, trimethoprim, gentamicin, Enrofloxacin, Cefotaxime and
ciprofloxacin.
The pH and chloride levels of the affected quarter milk in subclinical and
clinical mastitis cases before the treatment showed significant increase compared
to the nonaffected quarter milk values. These values showed significant decrease
to normal level on post treatment.

The mean values of citric acid, lactose and calcium levels of the affected
quarter milk in subclinical and clinical mastitis cases before the treatment,
showed significant decrease before treatment when compared to the nonaffected
quarter milk values. These values significantly increased and reached normal
levels after treatment.
Oral administration of tri-sodium citrate was found to be effective in
treating subclinical mastitis cases. All the biochemical parameters of milk came
to normal level in all cases in the trisodium citrate alone treated group by sixth
day of treatment and also the increase in milk production after the treatment was
slightly better in this group.
Oral administration of tri-sodium citrate along with antibiotic was found
to be effective in managing clinical mastitis cases. 100 percent bacteriological
cure was observed in this group whereas only 75per cent bacteriological cure
observed in the other group.