In vitro somatic embryogenesis in bael
By: Hima Sugathan.
Contributor(s): Sulekha G R (Guide).
Material type:
BookPublisher: Vellayani Department of Plantation Crops and Spices, College of Agriculture 2003DDC classification: 633.8 Online resources: Click here to access online Dissertation note: MSc Abstract: Standardization of techniques for the in vitro propagation of bael
[Aegle marmelos (L.) Corr.] via somatic embryogenesis was attempted. The
studies were carried out at the Plant Molecular Biology and Biotechnology
Centre, College of Agriculture, Vellayani, during 2001-2003. Attempts
were made to induce somatic embryogenesis using explants such as
cotyledons, nucellus, hypocotyls, integuments, in vitro leaves, roots,
internode and ex-vitro leaves. The effects of culture medium (basal media,
major and minor nutrients, plant growth substances, sucrose, coconut water,
activated charcoal and malt extract), culture conditions and frequency of
subculture on various stages of somatic embryogenesis were studied.
Among the various explants tried, somatic embryogenesis could be
induced only from cotyledons. Induction of embryogenic calli from
cotyledonary explants occurred in MS basal medium supplemented with 2,4 0
0.2 mg r', BA 0.1 mg r', CW 200.0 ml r', sucrose 40.0 g r' and agar 6.0 g r'.
But the percentage initiation was low (16.67 per cent). Dark culture
condition was found to favour callus initiation. Sub culturing in the medium
of same composition at an interval of 15 days increased the percentage
induction of callus by 20.00 than when subcultured at 10 days interval.
Liquid culture with filter paper bridges induced organogenesis than
embryogenesis.
The embryogenic calli obtained in the induction medium, when
sub cultured in half strength MS basal medium supplemented with BA 0.2
mg i', sucrose 40.0 g i', and agar 6.0 g r' initiated 40-50 globular somatic
embryos. Even after two months, the somatic embryos did not germinate.
However, they are kept in the same medium for observing further growth
and development.
Further studies on refinement of culture media and culture conditions
are necessary for evolving a reliable protocol for somatic embryogenesis in
Aegle marmelos.
| Item type | Current location | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|
Theses
|
KAU Central Library, Thrissur Theses | 633.8 HIM/IN (Browse shelf) | Available | 172224 |
MSc
Standardization of techniques for the in vitro propagation of bael
[Aegle marmelos (L.) Corr.] via somatic embryogenesis was attempted. The
studies were carried out at the Plant Molecular Biology and Biotechnology
Centre, College of Agriculture, Vellayani, during 2001-2003. Attempts
were made to induce somatic embryogenesis using explants such as
cotyledons, nucellus, hypocotyls, integuments, in vitro leaves, roots,
internode and ex-vitro leaves. The effects of culture medium (basal media,
major and minor nutrients, plant growth substances, sucrose, coconut water,
activated charcoal and malt extract), culture conditions and frequency of
subculture on various stages of somatic embryogenesis were studied.
Among the various explants tried, somatic embryogenesis could be
induced only from cotyledons. Induction of embryogenic calli from
cotyledonary explants occurred in MS basal medium supplemented with 2,4 0
0.2 mg r', BA 0.1 mg r', CW 200.0 ml r', sucrose 40.0 g r' and agar 6.0 g r'.
But the percentage initiation was low (16.67 per cent). Dark culture
condition was found to favour callus initiation. Sub culturing in the medium
of same composition at an interval of 15 days increased the percentage
induction of callus by 20.00 than when subcultured at 10 days interval.
Liquid culture with filter paper bridges induced organogenesis than
embryogenesis.
The embryogenic calli obtained in the induction medium, when
sub cultured in half strength MS basal medium supplemented with BA 0.2
mg i', sucrose 40.0 g i', and agar 6.0 g r' initiated 40-50 globular somatic
embryos. Even after two months, the somatic embryos did not germinate.
However, they are kept in the same medium for observing further growth
and development.
Further studies on refinement of culture media and culture conditions
are necessary for evolving a reliable protocol for somatic embryogenesis in
Aegle marmelos.
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