Seroepidemiology of Bovine Brucellosis
By: Priya P.
Contributor(s): Vijayakumar K (Guide).
Material type:
BookPublisher: Mannuthy Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences 2003DDC classification: 636.0894 Online resources: Click here to access online Dissertation note: MVSc Abstract: An investigation was carried out on 1602 cattle and buffaloes of Kerala
including 1535 cattle and 67 buffaloes to assess the seroprevalence of brucellosis
using serological tests like Rose Bengal plate' test, Standard tube agglutination
test, 2-Mercaptoethanol test and Avidin-Biotin ELISA. Sera samples were
collected from bovines belonging to different farms, hospitals and households of
Kerala covering eleven districts. Samples were collected randomly from animals
of different sex, age, breed, gestation, lactation, reproductive performance,
management practices and also from slaughter house. Out of the 1602 sera
samples tested 15.29 per cent gave positive result for brucella antibodies. Of this
cattle showed a seropositivity of 14.72 per cent and buffaloes showed 28.35 per
cent seropositivity. Female cattle and female buffaloes showed higher
seroprevalence than male cattle and male buffaloes. Bulls and cows aged more
than five years showed the highest seropositivity. Among the breeds pure bred
Holstein Friesian bulls and crossbred Jersey female cattle showed the highest
seroprevalence. Seroprevalence of brucellosis was found higher in pregnant
cattle, in late stage of gestation, in pregnant dry animals and also in cows and
heifers with the history of repeat breeding. Animals maintained under
unorganised farming system, animals from high altitude agroecological region
showed more number of positive reactors. Northern zone of Kerala showed more
number of positive reactors. Districtwise analysis revealed that animals
belonging to Kozhikode district having highest seropositivity and animals from
Kottayam and Malappuram districts as zero positives. RBPT, STA T and 2 MET
was evaluated for the sensitivity, specificity, predictive values and accuracy
using, A-B ELISA as the 'gold standard'. RBPT was found to have higher
sensitivity, predictive value of a negative test and accuracy than STAT and 2
MET and all the three tests found to have 100 per cent specificity and predictive
value of a positive test with that of ELISA. It was concluded that RBPT can be
used as a preliminary screening test and ELISA as a confirmatory diagnostic test.
| Item type | Current location | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|
Theses
|
KAU Central Library, Thrissur Theses | 636.0894 PRI/SE (Browse shelf) | Available | 172236 |
MVSc
An investigation was carried out on 1602 cattle and buffaloes of Kerala
including 1535 cattle and 67 buffaloes to assess the seroprevalence of brucellosis
using serological tests like Rose Bengal plate' test, Standard tube agglutination
test, 2-Mercaptoethanol test and Avidin-Biotin ELISA. Sera samples were
collected from bovines belonging to different farms, hospitals and households of
Kerala covering eleven districts. Samples were collected randomly from animals
of different sex, age, breed, gestation, lactation, reproductive performance,
management practices and also from slaughter house. Out of the 1602 sera
samples tested 15.29 per cent gave positive result for brucella antibodies. Of this
cattle showed a seropositivity of 14.72 per cent and buffaloes showed 28.35 per
cent seropositivity. Female cattle and female buffaloes showed higher
seroprevalence than male cattle and male buffaloes. Bulls and cows aged more
than five years showed the highest seropositivity. Among the breeds pure bred
Holstein Friesian bulls and crossbred Jersey female cattle showed the highest
seroprevalence. Seroprevalence of brucellosis was found higher in pregnant
cattle, in late stage of gestation, in pregnant dry animals and also in cows and
heifers with the history of repeat breeding. Animals maintained under
unorganised farming system, animals from high altitude agroecological region
showed more number of positive reactors. Northern zone of Kerala showed more
number of positive reactors. Districtwise analysis revealed that animals
belonging to Kozhikode district having highest seropositivity and animals from
Kottayam and Malappuram districts as zero positives. RBPT, STA T and 2 MET
was evaluated for the sensitivity, specificity, predictive values and accuracy
using, A-B ELISA as the 'gold standard'. RBPT was found to have higher
sensitivity, predictive value of a negative test and accuracy than STAT and 2
MET and all the three tests found to have 100 per cent specificity and predictive
value of a positive test with that of ELISA. It was concluded that RBPT can be
used as a preliminary screening test and ELISA as a confirmatory diagnostic test.
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