Prevalence of leptospirosis in animals in and around Thrissur
By: Manju Soman.
Contributor(s): Jayaprakasan V (Guide).
Material type:
BookPublisher: Mannuthy Department of Microbiology, College of Veterinary and Animal Sciences 2004DDC classification: 636.0896 Online resources: Click here to access online Dissertation note: MVSc Abstract: A study was undertaken to assess the prevalence of leptospirosis in animals
and man, in and around Thrissur.
A total of 501 sera samples, collected from dogs, cattle, pigs, rodents
(bandicoots and rats) and human beings were subjected to serologic testing, for
detection of Leptospira specific antibodies, by MAT, PHA and indirect IgG
ELISA. Isolation of Leptospira was tried from blood of clinically suspected cases
of human and canine leptospirosis and from kidney tissues of rodents.
The study revealed the presence of antibodies against Leptospira in human
beings and all the four species of animals examined by the three tests employed.
The prevalence rates detected by MAT in canines, bovines, porcines, murines and
human beings were 36.36 per cent, 47 per cent, 23.8 per cent, 21.42 per cent and
54.54 per cent respectively.
Out of 30 human blood samples subjected to isolation trials in
Fletcher'slEMJH semisolid media with enrichment, Leptospira could be isolated
from a single human patient, in Fletcher's semisolid medium with 10 per cent
rabbit serum. Of the 35 rodent kidney tissues tried for isolation in Fletcher's semi
solid media, leptospires were isolated from three bandicoots and two rats.
Indirect ELISA was found to be most sensitive, of the three tests,for rapid
screening of the population for leptospirosis, while PHA was found to be a fairly
good test for diagnosis of acute leptospirosis. The MAT proved to be the most
specific test which could also identify the serogroup identity of the infecting
Leptospira. Leptospira pomona and L. australis were detected by MAT as the
common serogroups prevalent in man, animals and rodents in this area.
The prevalence of common leptospiral serovars in animals and man,
indicated that human beings which were the end hosts for Leptospira could have
acquired the infection mostly from animals like dogs, cattle and pigs, while the
isolation ofleptospires from rodents revealed the carrier status of these animals.
| Item type | Current location | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|
Theses
|
KAU Central Library, Thrissur Theses | 636.0896 MAN/PR (Browse shelf) | Available | 172255 |
MVSc
A study was undertaken to assess the prevalence of leptospirosis in animals
and man, in and around Thrissur.
A total of 501 sera samples, collected from dogs, cattle, pigs, rodents
(bandicoots and rats) and human beings were subjected to serologic testing, for
detection of Leptospira specific antibodies, by MAT, PHA and indirect IgG
ELISA. Isolation of Leptospira was tried from blood of clinically suspected cases
of human and canine leptospirosis and from kidney tissues of rodents.
The study revealed the presence of antibodies against Leptospira in human
beings and all the four species of animals examined by the three tests employed.
The prevalence rates detected by MAT in canines, bovines, porcines, murines and
human beings were 36.36 per cent, 47 per cent, 23.8 per cent, 21.42 per cent and
54.54 per cent respectively.
Out of 30 human blood samples subjected to isolation trials in
Fletcher'slEMJH semisolid media with enrichment, Leptospira could be isolated
from a single human patient, in Fletcher's semisolid medium with 10 per cent
rabbit serum. Of the 35 rodent kidney tissues tried for isolation in Fletcher's semi
solid media, leptospires were isolated from three bandicoots and two rats.
Indirect ELISA was found to be most sensitive, of the three tests,for rapid
screening of the population for leptospirosis, while PHA was found to be a fairly
good test for diagnosis of acute leptospirosis. The MAT proved to be the most
specific test which could also identify the serogroup identity of the infecting
Leptospira. Leptospira pomona and L. australis were detected by MAT as the
common serogroups prevalent in man, animals and rodents in this area.
The prevalence of common leptospiral serovars in animals and man,
indicated that human beings which were the end hosts for Leptospira could have
acquired the infection mostly from animals like dogs, cattle and pigs, while the
isolation ofleptospires from rodents revealed the carrier status of these animals.
There are no comments for this item.