MSc

An experiment entitled "Impact ofbio agents and soil amendments on the
performance of patchouli" was conducted at the Department of Plantation crops and
Spices, College of Horticulture, Vellanikkara during 2002-04 to elucidate the effect
of application of bioagents, soil amendments and bioregulators on growth, yield and
oil quality of patchouli. The study was also aimed at refming vegetative propagation
technique to generate disease free quality planting materials. The investigations were
conducted in two experiments.
Results of the first experiment, " Impact of bioagents on the production
of quality planting materials in the nursery "revealed that bioagents had a significant
influence on the growth characters of the crop in the nursery stage wherein
significant differences were observed among the treatments with respect to
characters such as number of leaves, number of roots, length of roots and root
volume. Root characters were markedly influenced by bioagents and bioregulators.
Root length and root volume were found the highest for the treatment receiving
combined application of Trichoderma and Pseudomonas fluorescens at the rate of 2
g each at the time of planting. This treatment also recorded earliest sprout
emergence in 6.3 days after planting the terminalcuttings in the nursery.
Terminal cuttings which were treated with IBA at the rate of 1000 ppm at
the time of planting came to rooting earlier in 8 days after planting in the nursery.
Root number was also recorded the highest with the same treatment.
The highest leaf number in 45 days old patchouli plants was recorded in
nursery with the incorporation of Trichoderma at the rate of 2 g per bag. This
treatment also recorded the highest sprout length of 0.45 cm.
Earliness in sprouting, length of sprout and earliness in rooting were not
found influenced by bioagents in nursery experiment.
Plants raised in the nursery by the combined application of Trichoderma
and Pseudomonas fluorescens rated the best in the nursery were selected for main
field experiment. Impact of bioagents and soil amendments on growth, yield and oil
quality were studied in the main field and results obtained revealed that treatments

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involving combined application of bioagents and soil amendments significantly
influenced the growth and yield parameters of the crop plant
..
Plant height was the highest with the combined application of lime, neem
cake, Trichoderma and Pseudomonas fluorescens along with the application of
recommended doses of FYM and NPK both in main crop and regenerated crop.
Compared to control, this treatment also improved vegetative characters like number
of leaves and yield attributes like fresh herb yield and fresh leaf yield both in main
crop and regenerated crop.
Combined application of control, lime, neem cake and Pseudomonas
fluorescens along with the recommended doses of FYM and NPK recorded highest
number of branches and greatest plant spread. Leaf area also was found varying
significantly among treatments. Application of neem cake, Trichoderma and
Pseudomonas fluorescens in addition to the recommended doses of FYM and NPK
recorded the highest leaf area. Dry leaf yield recorded was also the highest with the
same treatment.
Oil yield and oil content in the plant were found to be significantly
influenced by bioagents and soil amendments. The plants treated with a combination
of neem cake + Trichoderma + Pseudomonas fluorescens along with the
recommended doses of FYM and NPK recorded highest values of oil yield and oil
content.
Physicochemical properties of the essential oil of patchouli were
analysed wherein it was, observed that specific gravity of oil between 0.940 and
0.953 and refractive index was recorded between 1.5032 and 1.5150 at 31 Qc.
Gas chromatographic profile of oil samples were analysed and significant
variation with respect to the application of various treatments was observed. Number
of components present in the oil sample varied significantly among treatments. It is
observed that when the number of peaks increases, the percentage of major
components in the oil decreases. Treatments receiving neem cake along with NPK
and FYM recorded maximum number of components in the oil sample. It is found
that neem cake can add optimum level of nutrients to the soil at a slow and steady
rate which finally resulted in the increment of oil components.