Management of biodegradable plant tissue culture lab wastes through biomethanogenesis
By: Abdulla Fayas T.
Contributor(s): Rajendran P C(Guide).
Material type:
BookPublisher: Vellanikkara Centre For Plant Biotechnology and Molecular Biology, College of Horticulture 2008DDC classification: 660.6 Online resources: Click here to access online Dissertation note: MSc Abstract: Generating renewable source of energy from tissue culture laboratory
waste by the process of biomethanogenesis is the focal theme of present
investigation. Unlike developed countries, the developing countries are hesitant to
establish more number of biotechnology/ tissue culture laboratories due to
financial constraints. Easy and regular availability of biogas from TC wastes will
be a boon to establish self-sustainable TC laboratory in view of present energy
The biogas experimental units required for the study was designed and
various treatments were employed for the biodegradation of tissue culture waste,
using the methanogenic bacteria Methanobacterium ruminatium,
Methanobacterium formicicum; Methanosarcina barkeri, Bactereoides
ruminicola, Selenomonas ruminatium, Eubacterium tortuosum and Clostridium
butyricum. Treatment involving TC waste and cow dung was also conducted for
biomethanation in the present study. Quantity of gas production and its
combustibility was noticed for various treatments. In bacterial treatments the
quantity of gas generation was highest for Clostridium butyricum. Only treatments
involving cow dung produced combustible gas.
Molecular characterization, of methanogenic bacterial cultures was also
done for finding the genetic similarity between them. RAPD followed by scoring
. of the bands by UPGA routine showed maximum similarity between bacterial
cultures of Methanobacterium ruminatium and Methanobacterium jormicicum
with Methanosarcina barkeri.
Physio-chemical characters like CIN ratio of the TC wastes, pH and
temperature of medium and Hydraulic retention time was also observed for the
various treatments. The CIN ratio of the TC wastes was found to be very low and
nowhere near the optimum CIN ratio of 20-30 required for gas production. Other
parameters like pH of the treatments and Hydraulic retention time was also.
• noticed. The pH of the treatments involving bacterial cultures was very low,
considering the normal pH of 6.8 to 7.5 required in biogas generation.
The main constraints in the biogas generation were found out to be the low
CIN ratio of the TC waste and the low pH of the medium. The present study
indicated the possibility ofbio-gas generation from TC waste through fortification
using various supplements like coconut water and coir pith which have higher
CIN ratio.
| Item type | Current location | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|
Theses
|
KAU Central Library, Thrissur Theses | 660.6 ABD/MA (Browse shelf) | Available | 172777 |
MSc
Generating renewable source of energy from tissue culture laboratory
waste by the process of biomethanogenesis is the focal theme of present
investigation. Unlike developed countries, the developing countries are hesitant to
establish more number of biotechnology/ tissue culture laboratories due to
financial constraints. Easy and regular availability of biogas from TC wastes will
be a boon to establish self-sustainable TC laboratory in view of present energy
The biogas experimental units required for the study was designed and
various treatments were employed for the biodegradation of tissue culture waste,
using the methanogenic bacteria Methanobacterium ruminatium,
Methanobacterium formicicum; Methanosarcina barkeri, Bactereoides
ruminicola, Selenomonas ruminatium, Eubacterium tortuosum and Clostridium
butyricum. Treatment involving TC waste and cow dung was also conducted for
biomethanation in the present study. Quantity of gas production and its
combustibility was noticed for various treatments. In bacterial treatments the
quantity of gas generation was highest for Clostridium butyricum. Only treatments
involving cow dung produced combustible gas.
Molecular characterization, of methanogenic bacterial cultures was also
done for finding the genetic similarity between them. RAPD followed by scoring
. of the bands by UPGA routine showed maximum similarity between bacterial
cultures of Methanobacterium ruminatium and Methanobacterium jormicicum
with Methanosarcina barkeri.
Physio-chemical characters like CIN ratio of the TC wastes, pH and
temperature of medium and Hydraulic retention time was also observed for the
various treatments. The CIN ratio of the TC wastes was found to be very low and
nowhere near the optimum CIN ratio of 20-30 required for gas production. Other
parameters like pH of the treatments and Hydraulic retention time was also.
• noticed. The pH of the treatments involving bacterial cultures was very low,
considering the normal pH of 6.8 to 7.5 required in biogas generation.
The main constraints in the biogas generation were found out to be the low
CIN ratio of the TC waste and the low pH of the medium. The present study
indicated the possibility ofbio-gas generation from TC waste through fortification
using various supplements like coconut water and coir pith which have higher
CIN ratio.
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