Evaluation of hepatoprotective effect of ethanolic extract of eugenia jamolana (njaval) leaves on paracetamol induced toxicity in rats
By: Midhun M V.
Contributor(s): Aravindakshan C M (Guide).
Material type:
BookPublisher: Mannuthy Department of Pharmacology and Toxicology, College of Veterinary and Animal Sciences 2008DDC classification: 636.089 Online resources: Click here to access online Dissertation note: MVSc Abstract: The present study is undertaken to evaluate the hepatoprotective effect of Eugenia jambolana (Njaval) leaves on paracetamol induced toxicity in rats.
Fourty adult male albino wistar rats weighing 150-200 g were randomly divided into five groups of eight animals each. Group I received 5 ml/Kg/day of 3 % gum acacia (vehicle) orally daily for ten days. Groups III and IV received ethanolic extract of Eugenia jambolana leaves at the dose rate of 100 and 200 mg/Kg/day respectively in 3% gum acacia for ten days. Group V received silymarin 100 mg/Kg/day in 3% gum acacia for ten days. Hepatotoxicity was induced in groups II, III, IV and V by giving a single dose of paracetamol at the dose of 2g/Kg on the eighth day of the experiment. Blood was collected before and after the experiment for various biochemical and haematological parameters. Body weight of all the animals was recorded on the first day and last day. On the tenth day all the animals were sacrificed and liver was collected for biochemical and histopathological studies.
Paracetamol treated rats showed a decrease in their body weight. Biochemical parameters like superoxide dismutase and catalase were also decreased in the paracetamol treated groups when compared with the others. Liver marker enzymes like ALT, AST were increased in the paracetamol treated groups, but the treatment with extract at both the doses reduced the elevated level of these enzymes. The total protein level and albumin level were decreased in the paracetamol treated groups and a gradual increase was noticed in the extract treated groups. The total bilirubin level was highest in the paracetamol alone treated group and less in the extract treated groups. Haematological parameters showed not much significant change with the treatment.
Phytochemical analysis of the plant extract revealed the presence of phenolic compounds, flavonoids, tannins, glycosides, diterpenes and triterpenes. All the animals showed normal liver except the paracetamol treated group. In the paracetamol treated group areas of necrosis was noticed. On histological examination, the paracetamol treated group showed areas of coagulation necrosis and the treatment groups showed regeneration of the hepatocytes.
The study revealed that the ethanolic extract of leaves of E. jambolana at the doses 100 and 200 mg/Kg/day have significant hepatoprotective activity in paracetamol induced toxicity in rats.
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Theses
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KAU Central Library, Thrissur Theses | 636.089 MID/EV (Browse shelf) | Available | 172816 |
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MVSc
The present study is undertaken to evaluate the hepatoprotective effect of Eugenia jambolana (Njaval) leaves on paracetamol induced toxicity in rats.
Fourty adult male albino wistar rats weighing 150-200 g were randomly divided into five groups of eight animals each. Group I received 5 ml/Kg/day of 3 % gum acacia (vehicle) orally daily for ten days. Groups III and IV received ethanolic extract of Eugenia jambolana leaves at the dose rate of 100 and 200 mg/Kg/day respectively in 3% gum acacia for ten days. Group V received silymarin 100 mg/Kg/day in 3% gum acacia for ten days. Hepatotoxicity was induced in groups II, III, IV and V by giving a single dose of paracetamol at the dose of 2g/Kg on the eighth day of the experiment. Blood was collected before and after the experiment for various biochemical and haematological parameters. Body weight of all the animals was recorded on the first day and last day. On the tenth day all the animals were sacrificed and liver was collected for biochemical and histopathological studies.
Paracetamol treated rats showed a decrease in their body weight. Biochemical parameters like superoxide dismutase and catalase were also decreased in the paracetamol treated groups when compared with the others. Liver marker enzymes like ALT, AST were increased in the paracetamol treated groups, but the treatment with extract at both the doses reduced the elevated level of these enzymes. The total protein level and albumin level were decreased in the paracetamol treated groups and a gradual increase was noticed in the extract treated groups. The total bilirubin level was highest in the paracetamol alone treated group and less in the extract treated groups. Haematological parameters showed not much significant change with the treatment.
Phytochemical analysis of the plant extract revealed the presence of phenolic compounds, flavonoids, tannins, glycosides, diterpenes and triterpenes. All the animals showed normal liver except the paracetamol treated group. In the paracetamol treated group areas of necrosis was noticed. On histological examination, the paracetamol treated group showed areas of coagulation necrosis and the treatment groups showed regeneration of the hepatocytes.
The study revealed that the ethanolic extract of leaves of E. jambolana at the doses 100 and 200 mg/Kg/day have significant hepatoprotective activity in paracetamol induced toxicity in rats.
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