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Exploitation of bisexual varient in developing high yielding types of piper longum L.

By: Kanimozhi K.
Contributor(s): Sujatha V S (Guide).
Material type: materialTypeLabelBookPublisher: Vellanikkara Department of Plantation Crops and Spices, College of Horticulture 2010DDC classification: 633.8 Online resources: Click here to access online Dissertation note: MSc Abstract: Piper longum L. is a dioecious medicinal species. Mature female spike is the officinal part. However, female spikes are small (2.0 - 3.0 cm) compared to male (6.0 - 7.5 cm). A bisexual variant (Acc.P25) was identified in P. longum (Sujatha and Nybe, 2007) with spikes as long as male spikes. The present investigation was undertaken with the objective of studying the reproductive biology of Piper longum L. and for developing long spiked high yielding varieties of Piper longum L. Reproductive biology Spikes were cylindrical and creamy white in female. In male and bisexual types, immature spikes were green, changing to dull yellow on maturity. Time taken for attaining full length of spike was 22 days in female, 43 days in male and 46 days in bisexual types. Anthesis and anther dehiscence were between 7.30 am and 4.30 pm with a peak between 10.30 am to 12.30 pm. Pollen fertility was maximum at 9.30 am (42.54 per cent). Complete opening of flowers in an inflorescence took seven days in male, female and bisexual types. Complete dehiscence of anthers also took one week in male and bisexual inflorescences. Developing bisexual types Effect of growth regulators in developing fully bisexual inflorescences in Acc.P25 indicated that GA3 (5 ppm, 10 ppm and 50 ppm), BA (100 ppm and 500 ppm) and Boron 3 ppm could induce fully bisexual spikes. Different methods of pollination showed that dry method was the most effective for getting seed set in P. longum L. In selfing and crossing experiments, seed set could be obtained only in crosses involving female type as female parent and bisexual type as male parent. Maximum seed set was obtained during September, under dry method of pollination. Seeds germinated in 21- 69 days. Cotyledonary leaves opened in 2 to31 days. The first true leaves opened in 2 to 41 days.
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Theses
633.8 KAN/EX PG (Browse shelf) Available 172981

MSc

Piper longum L. is a dioecious medicinal species. Mature female spike is the officinal part. However, female spikes are small (2.0 - 3.0 cm) compared to male (6.0 - 7.5 cm). A bisexual variant (Acc.P25) was identified in P. longum (Sujatha and Nybe, 2007) with spikes as long as male spikes.
The present investigation was undertaken with the objective of studying the reproductive biology of Piper longum L. and for developing long spiked high yielding varieties of Piper longum L.
Reproductive biology
Spikes were cylindrical and creamy white in female. In male and bisexual types, immature spikes were green, changing to dull yellow on maturity. Time taken for attaining full length of spike was 22 days in female, 43 days in male and 46 days in bisexual types. Anthesis and anther dehiscence were between 7.30 am and 4.30 pm with a peak between 10.30 am to 12.30 pm.
Pollen fertility was maximum at 9.30 am (42.54 per cent). Complete opening of flowers in an inflorescence took seven days in male, female and bisexual types. Complete dehiscence of anthers also took one week in male and bisexual inflorescences.
Developing bisexual types
Effect of growth regulators in developing fully bisexual inflorescences in Acc.P25 indicated that GA3 (5 ppm, 10 ppm and 50 ppm), BA (100 ppm and 500 ppm) and Boron 3 ppm could induce fully bisexual spikes.
Different methods of pollination showed that dry method was the most effective for getting seed set in P. longum L. In selfing and crossing experiments, seed set could be obtained only in crosses involving female type as female parent and bisexual type as male parent. Maximum seed set was obtained during September, under dry method of pollination. Seeds germinated in 21- 69 days. Cotyledonary leaves opened in 2 to31 days. The first true leaves opened in 2 to 41 days.

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