Beta lactoglobulin polymorphism in goats of Kerala
By: Sudina K.
Contributor(s): Bindu K.A (Guide).
Material type:
BookPublisher: Mannuthy Department of Animal Breeding, Genetics and Biostatistics, College of Veterinary and Animal Sciences 2010DDC classification: 636.082 Online resources: Click here to access online Dissertation note: MVSc Abstract: Βeta-lactoglobulin gene polymorphism and its association with various milk production traits were investigated in DNA samples isolated from blood of 20 Malabari, 25 Attappady black 30 crossbred goats of University goat farm and 45 Malabari goats from the native tract of Malabari breed. Study was conducted by PCR-RFLP using Sac II restriction endonuclease.
Digestion of the amplified PCR product with Sac II restriction endonuclease revealed three genotypes (S1S1, S1S2 and S2S2) in Malabari, Attappady black and crossbred goats, indicating the presence of S1 (349 and 77 bp fragments) and S2 (426 bp fragment) alleles. The S1/S2 allele frequencies were 0.37/0.63, 0.34/0.66 and 0.18/0.82, respectively in Malabari, Attappady black and crossbred goats. The genotypes of β-LG/Sac II polymorphism were distributed according to Hardy-Weinberg equilibrium with frequencies 0.04 (S1S1), 0.60 (S1S2) and 0.36 (S2S2) in Attappady black and 0.10 (S1S1), 0.17 (S1S2) and 0.73 (S2S2) in crossbred goats under study. But the Malabari goat population under study with genotype frequencies 0.14 (S1S1), 0.46 (S1S2) and 0.40 (S2S2), was found to violate H-W equilibrium. So this population was further classified and genotypic frequencies of subpopulations like Tellicherry (0.14, 0.50, 0.36), Badagara (0.2, 0.25, 0.55) and University farm (0.09, 0.61, 0.30) populations were in Hardy-Weinberg equilibrium.
From the present study a significant association could be detected between β-LG polymorphism and peak milk yield in Malabari breed but not in other groups. Malabari goats carrying S2 allele showed a significantly higher average (p<0.05) (420±74.24 ml and 501.79±46.47 ml) compared to animals homozygous for S1 allele (250±13.36 ml). A statistically significant association could not be established between β-LG polymorphism and other milk production traits. So the introduction of this polymorphism can complement current selection programme.
| Item type | Current location | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|
Theses
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KAU Central Library, Thrissur Theses | 636.082 SUD/BE PG (Browse shelf) | Available | 173003 |
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MVSc
Βeta-lactoglobulin gene polymorphism and its association with various milk production traits were investigated in DNA samples isolated from blood of 20 Malabari, 25 Attappady black 30 crossbred goats of University goat farm and 45 Malabari goats from the native tract of Malabari breed. Study was conducted by PCR-RFLP using Sac II restriction endonuclease.
Digestion of the amplified PCR product with Sac II restriction endonuclease revealed three genotypes (S1S1, S1S2 and S2S2) in Malabari, Attappady black and crossbred goats, indicating the presence of S1 (349 and 77 bp fragments) and S2 (426 bp fragment) alleles. The S1/S2 allele frequencies were 0.37/0.63, 0.34/0.66 and 0.18/0.82, respectively in Malabari, Attappady black and crossbred goats. The genotypes of β-LG/Sac II polymorphism were distributed according to Hardy-Weinberg equilibrium with frequencies 0.04 (S1S1), 0.60 (S1S2) and 0.36 (S2S2) in Attappady black and 0.10 (S1S1), 0.17 (S1S2) and 0.73 (S2S2) in crossbred goats under study. But the Malabari goat population under study with genotype frequencies 0.14 (S1S1), 0.46 (S1S2) and 0.40 (S2S2), was found to violate H-W equilibrium. So this population was further classified and genotypic frequencies of subpopulations like Tellicherry (0.14, 0.50, 0.36), Badagara (0.2, 0.25, 0.55) and University farm (0.09, 0.61, 0.30) populations were in Hardy-Weinberg equilibrium.
From the present study a significant association could be detected between β-LG polymorphism and peak milk yield in Malabari breed but not in other groups. Malabari goats carrying S2 allele showed a significantly higher average (p<0.05) (420±74.24 ml and 501.79±46.47 ml) compared to animals homozygous for S1 allele (250±13.36 ml). A statistically significant association could not be established between β-LG polymorphism and other milk production traits. So the introduction of this polymorphism can complement current selection programme.
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