MSc.

An experiment was carried out at College of Agriculture, Vellayani to investigate the interactive effect of nitrogen and sulphur on their release pattern and use efficiency in ferralitic soils with bhindi as the test crop. The experiment consisted of an incubation study and a field experiment.
The incubation study was carried out to understand the release pattern of N and S from their sources viz. urea and gypsum in the soil at different sampling stages (30 and 100 days of incubation) using ferralitic soil kept at field capacity. The treatments consisted of M1N1S1, MINIS2 , M1N1S3 , M1N2S1 , M1N2S2 ,M1N2S3 ,M1N3S1 , M1N3S2 , M1N3S3 , M2N1S1 , M2N1S2 , M2N1S3 , M2N2S1 , M2N2S2 , M2N2S3 , M2N3S1 , M2N3S2 and M2N3S3 and M0N0S0 . Levels of N include N1 (50 kg ha-1), N2 (80 kg ha-1) and N3 (110 kg ha-1). M1 include P2O5 @ 8 kg ha-1+ K2O @ 25kg ha-1 (current POP) and M2 include P2O5 @ 35 kgha-1 + K2O @ 70 kg ha-1(modified POP). Different levels of S are S1 (10 kg ha-1), S2 (15 kg ha-1) and S3 (20 kg ha-1). Soil samples were collected and analyzed for pH, available N and available S content. There was increase in pH value to the neutral range at fifty per cent flowering stage (30 Days of Incubation) and all the treatments showed a decreasing trend in the pH value at 100 Days of Incubation. Available N and available S content increased to some extent at fifty per cent flowering and decreased at the final harvest stage. Application of N up to 110 kg ha-1 significantly increased the available N status of soil and S application up to 20 kg ha-1 increased the soil S status during incubation.
The field experiment was laid out in 2×3×3+1 factorial RBD having three replications using bhindi variety Varsha Uphar as the test crop. The treatments were similar to that of the incubation study.
S application @15 kg ha-1 significantly reduced the internodal length. N and S interaction N @ 80 kg ha-1 and S @ 15 kg ha-1 showed significant influence on fruit length. The maximum number of fruits and highest yield was obtained when the nutrients were applied @ 80 kg N ha-1 + 8 kg P2O5 ha-1 + 25 kg K2O ha-1 + 15 kg S ha-1. Considering the N and S interaction effects, combined application of N @ 80 kg ha-1 and S @ 20 kg ha-1 increased the number of fruits and yield. Application of N up to 80 kg ha-1 increased the crop yield and above this level, there was reduction in yield. The increase in number of fruits per plant by the application of N and S may be due to the highest uptake and efficient utilization of nutrients. N @ 110 kg ha-1 and S @ 20 kg ha-1 recorded the highest dry matter content. S application @ 15 kg ha-1 showed significant influence on N Use Efficiency (NUE) in bhindi. Application of 80 kg N ha-1 + 8 kg P2O5 ha-1 + 25 kg K2O ha-1 + 15 kg S ha-1 resulted in highest NUE.
By the application of urea and gypsum, there was slight increase in the soil pH. After N and S application, there was increase in organic carbon content of the soil. At fifty per cent flowering all the nutrients showed an increase in availability and there was a decrease at the final harvest stage because of crop uptake and various losses. N application @ 110 kg ha-1 significantly increased the available N status in the soil. S application up to 20 kg ha-1 significantly increased the available P K and S content in soil. As the levels S increased, there was increase in the exchangeable calcium in soil.
Higher levels of application of N recorded higher N content in both plant and fruit. Application of P and K at M2 (35 kg P2O5 ha-1 + 70 kg K2O ha-1) level significantly increased the content of P, K and Ca in plant and fruit. Increased S application increased the Ca content in plant since gypsum was used as the source of S. S application up to 20 kg ha-1 had significant influence on the fruit Ca and Mg concentration. The interactive effect of N and S was not significant in the case of S content in plant. But, N2S3 (N @ 80 kg ha-1 and S @ 20 kg ha-1) recorded the superior value. N application had got positive influence on the content of P, K, Ca, Mg and S in plant and fruit. By progressive increase in S application up to 20 kg ha-1and N application up to 80 kg ha-1, there was significant reduction in N: S ratio. N and S fertilization had significant influence on leaf chlorophyll content and application of 80 kg N ha-1, 35 kg P2O5 ha-1, 70 kg K2O ha-1 and 15 kg S ha-1 showed the superior value for chlorophyll content. An increasing trend was noticed with higher levels of N up to 80 kg ha-1 and S up to 20 kg ha-1. N and S application significantly enhanced the uptake of nutrients. Uptake of phosphorus, potassium, calcium, magnesium and S were highest when N, P, K and S were applied @ 80 kg N ha-1+ 35 kg P2O5 ha-1+ 70 kg K2O ha-1+ 20 kg S ha-1.
N application @ 80 kg ha-1 significantly influenced the B: C ratio. Among the treatments M1N2S2 (80 kg N ha-1 + 8 kg P2O5 ha-1 + 25 kg K2O ha-1 + 15 kg S ha-1) which gave the highest yield showed the highest B: C ratio. Maximum B: C ratio was obtained when N and S were applied @ 80 kg ha-1 and 15 kg ha-1 respectively. As the levels of S increased, B: C ratio also increased. Fertilization of N, P, K and S @ 80 kg ha-1, 35 kg ha-1, 70 kg ha-1 and 15 kg ha-1 respectively recorded the minimum Percentage Disease Incidence (yellow vein mosaic).
Among the treatments, yield, number of fruits per plant, N Use Efficiency and B: C ratio were highest for M1N2S2 (80 kg N ha-1 + 8 kg P2O5 ha-1 + 25 kg K2O ha-1 + 15 kg S ha-1) and it can be considered as the best treatment combination. Application of N significantly increased the yield and the yield was highest at N2 level (80 kg N ha-1). There was reduction in yield if we apply N @ 110 kg ha-1. By the application of S along with N increased the NUE and we can reduce the dose of N to 80 kg ha-1 instead of 110 kg N ha-1 for bhindi.