Temperature stress tolerance in small cardamom (Elettaria cardamomum Maton) upon root colonization by endophytic fungus Piriformospora indica
By: Chippy V B.
Contributor(s): K K Sabu (Guide).
Material type:
BookPublisher: Vellayani Department of Plant Biotechnology, College of Agriculture 2022Description: 86p.Subject(s): Plant BiotechnologyDDC classification: 660.6 Dissertation note: BSc - MSc (Int.) Summary: Elettaria cardamomum Maton, one of the most valuable and ancient spice crops, is
generally vulnerable to abiotic stress factors. The major factor that contributes to this
specificity of the plant is the temperature. Piriformospora indica symbiosis with
cardamom reduces the negative effects of temperature stress. This study was aimed to
identify temperature stress tolerant genes in P. indica co-cultivated cardamom plants
and evaluation of chlorophyll and mineral content.
P. indica colonized cardamom plants already established in JNTBGRI experimental
field for a period of 1.5 years were exposed to temperature stress (ambient temperature).
The changes in plant phenotypes and biochemical parameters were measured. To identify
temperature stress tolerant genes conferred by P. indica, the leaf transcriptome of
colonized and uncolonized plants were analyzed after 24h of temperature stress
induction.
Results obtained showed that colonization of cardamom roots by P. indica revealed
significant variation in leaf number, leaf length and tiller number of temperature stressed
plants compared to control (p < 0.05) for a period of 6months at 30 days intervals. In
addition, chlorophyll a, b, a + b and carotenoid contents were increased in the leaves of
the colonized plants under temperature stress conditions improving photosynthetic
efficiency. Mineral estimation revealed that plants treated with P. indica increased the
accumulation of Ca and Mg elements. Furthermore, the expression of the antioxidant
enzymes catalase, ascorbate peroxidase and superoxide dismutase were significantly upregulated by 4.83, 4.12 and 4.67 folds respectively in P. indica inoculated cardamom
plants during temperature stress. This improvement in the transcripts of antioxidant
enzyme genes (Catalase [CAT], Ascorbate peroxidase [APX] and Superoxide dismutase
[SOD] could be correlated with a significant increment in antioxidant enzyme content. In
conclusion, P. indica has the potential to enhance the cardamom growth under
temperature stress and alters the activities of enzymatic and non-enzymatic antioxidants
100
and chlorophyll content which would help them to grow in high temperature zones
of Kerala.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode |
|---|---|---|---|---|---|---|
Theses
|
KAU Central Library, Thrissur Theses | Reference Book | 660.6 CHI/TE PG (Browse shelf) | Not For Loan | 175684 |
BSc - MSc (Int.)
Elettaria cardamomum Maton, one of the most valuable and ancient spice crops, is
generally vulnerable to abiotic stress factors. The major factor that contributes to this
specificity of the plant is the temperature. Piriformospora indica symbiosis with
cardamom reduces the negative effects of temperature stress. This study was aimed to
identify temperature stress tolerant genes in P. indica co-cultivated cardamom plants
and evaluation of chlorophyll and mineral content.
P. indica colonized cardamom plants already established in JNTBGRI experimental
field for a period of 1.5 years were exposed to temperature stress (ambient temperature).
The changes in plant phenotypes and biochemical parameters were measured. To identify
temperature stress tolerant genes conferred by P. indica, the leaf transcriptome of
colonized and uncolonized plants were analyzed after 24h of temperature stress
induction.
Results obtained showed that colonization of cardamom roots by P. indica revealed
significant variation in leaf number, leaf length and tiller number of temperature stressed
plants compared to control (p < 0.05) for a period of 6months at 30 days intervals. In
addition, chlorophyll a, b, a + b and carotenoid contents were increased in the leaves of
the colonized plants under temperature stress conditions improving photosynthetic
efficiency. Mineral estimation revealed that plants treated with P. indica increased the
accumulation of Ca and Mg elements. Furthermore, the expression of the antioxidant
enzymes catalase, ascorbate peroxidase and superoxide dismutase were significantly upregulated by 4.83, 4.12 and 4.67 folds respectively in P. indica inoculated cardamom
plants during temperature stress. This improvement in the transcripts of antioxidant
enzyme genes (Catalase [CAT], Ascorbate peroxidase [APX] and Superoxide dismutase
[SOD] could be correlated with a significant increment in antioxidant enzyme content. In
conclusion, P. indica has the potential to enhance the cardamom growth under
temperature stress and alters the activities of enzymatic and non-enzymatic antioxidants
100
and chlorophyll content which would help them to grow in high temperature zones
of Kerala.
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