TY  - BOOK
AU  - Renu Joseph 
AU  - Dr. V. S. Sujatha (Guide)
TI  - Isoenzyme Variation in Curcuma with Special Reference to Curcuma longa L
U1  - 633.8 
PY  - 1999///
CY  - Vellanikkara
PB  - Department Of Plantation Crops And Spices,College Of Horticulture
N2  - The study on 'Isoenzyme variation in Curcuma with special reference to 
C. longa was conducted in the Department of Plantation Crops and Spices and 
Biochemistry laboratory at College of Horticulture, Vellanikkara, Thrissur from 
October 1997 to June 1999. Eighteen species of genus Curcuma, including 
C. longa were included in the study. Thirty three accessions of C. longa were 
selected to represent 21 morphotypes of Velayudhan et al. (1994). Six released 
varieties were also studied. These were analysed for three enzymes viz. peroxidase, 
esterase and GOT using polyacrylamide gel electrophoresis. 
The selection of ideal part for electrophoresis for each enzyme was done 
based on observations of banding pattern of root, rhizome, sprout and different 
maturity stages of leaf. Sampling and analysis was done during three weeks after 
sprouting and just before senescence of leaf to standardise the stage of growth of 
plant. Mature leaf just before its senescence was selected for peroxidase and GOT 
analysis. Tender leaf three weeks after sprouting was found to be ideal for esterase. 
lsoenzyrne banding pattern of 39 genotypes of C. longa were studied for 
esterase, peroxidase and GOT. The similarity index among the genotypes ranged 
from 0.39 to one. The genotypes were classified into 21 groups with similarity index 
one among the members. Morphotypes M-I, M-2 and r'A-3 ofVelayudhan et 01 
(1994) formed one group with similarity index one. M-7 and M-8 were also grouped 
into one. Released varieties under study showed more similarity with first group of 
morphotypes. 
On grouping the [8 species of Curcuma based on isoenzyme similarity 
C zedoaria, C. aeruginosa, C. malabarica, C. comosa and C. cacsia formed one 
group, C. amada, C. syivatica, C. aromatica and C. liarita the second group and 
 
C. soloensis, C. brog and C montana the third group. Cultivated type C longo and 
other sessile tuberising species C. latifolia and C. raktakanta showed variable 
similarity with other species and stood independently. Stoloniferous type C. vamana 
showed more similarity with sessile tuberising species than with non-sessile 
tuberising species, Non-sessile tuberising species C. aurantiaca and 
C. pseudomontana stood individually showing their distinctness from rest of the 
species. 


UR  - http://krishikosh.egranth.ac.in/handle/1/5810103803
ER  -