TY  - BOOK
AU  - Geetha R 
AU  - Sreekumaran T (Guide)
TI  - Effect of different freezing rates on Canine Spermatozoa
U1  - 636.082 
PY  - 2000///
CY  - Mannuthy
PB  - Department of Animal Reproduction, College of Veterinary and Animal Sciences
N2  - The objective of the study was to find out the effect of 
different freezing rates on post thaw motility, livability and 
acrosomal damage of dog spermatozoa. A total of 36 ejaculates of 
good quality collected from SIX Dachshund dogs by digital 
manipulation were processed for freezing in Tris citric acid 
fructose egg yolk diluent containing four per cent glycerol. The 
processed semen samples were subjected to three different freezing 
protocols such as 4cm height above the liquid nitrogen level for 10 
minutes (Fast freezing), Scm for 15 minutes (Moderate freezing) 
and 12cm for 20 minutes (Slow freezing). 
The mean volume of sperm rich fractions was 
0.6S±0.03ml. The colour and consistency of sperm rich fractions 
were thin milky. The mean density of sperm rich fraction was 
DD(D) and mean pH was 6.63±O.02. The mean concentration of 
sperm rich fraction was 221±7.36 millions per ml and the average 
initial motility was found to be 75±O.93 per cent. The mean 
percentage of live sperm count, sperm abnormalities and acrosomal 
damage of spermatozoa was Sl.17±O.73, 5.23±O.29 and 2.32±O.25 
 
respectively. Significant (P<O.05) variation m livability, sperm 
abnormalities and acrosomal damage of spermatozoa was found 
between dogs. 
The average percentage of motility, live sperm count, 
sperm abnormalities and acrosomal damage of spermatozoa was 
70.41± 1.22, 75.63±O.65, 7.28±0.43 and 5.34±O.31 after dilution, 
58.75±1.34, 63.60±O.89, 10.04±O.32 and 10.13±0.41 after chilling 
and 47.78±1.59, 50.65±1.31, 11.79±O.36 and 16.20±O.57 after 
equilibration period respectively. There was significant (P<O.O I) 
reduction in sperm motility and livability and increase in sperm 
abnormalities and acrosomal damage of spermatozoa after dilution, 
chilling and equilibration period. Significant (P<O.O 1) difference 
was found between dogs for the above parameters. 
The percentage of post thaw motility of spermatozoa 
was significantly (P<O.O 1) higher in fast freezing rate (34.31±1.66) 
when compared to moderate (25.83±1.66) and slow (24.44±1.27) 
freezing rates. There was significantly (P<O.O 1) higher percentage 
of live sperms and lower percentage of sperm abnormalities in fast 
freezing rate than in moderate and slow freezing rates. Eventhough 
the percentage of acrosomal damage was not statistically 
 

( 
 
significant among fast, moderate and slow freezing rates, lower 
percentage of acrosomal damage was recorded in fast freezing rate. 
From this study it could be inferred that fast freezing 
in which the straws were frozen at to 4cm height above the liquid 
nitrogen level for 10 minutes was superior to moderate (8cm for 15 
minutes) and slow (12 cm for 20 minutes) freezing rates. 


UR  - http://krishikosh.egranth.ac.in/handle/1/5810104378
ER  -