TY  - BOOK
AU  - Priya P M
AU  - Krishnan Nair G (Guide)
TI  - Seroprevalence And Restriction Enzyme Analsis Of Egg Drop Syndrome Virus
U1  - 636.089 6 
PY  - 2000///
CY  - Mannuthy
PB  - Department of Microbiology, College of veterinary and Animal Sciences
N2  - In the present study, seroprevalence of EDS-76 was 
conducted in five districts of Kerala in duck and' 
chicken flocks using HI and ELISA. Out of 322 chicken 
 

 
and 281 duck sera samples screened, 
 

an overall 
 

 
incidence of 14.91 per cent and 26.69 per cent 
respectively were recorded. The high proportion of 
birds showing antibodies to EDS-76 reveals that the 
infection is widespread in Kerala and may be the major 
 

 
etiological 
 

factor 
 

associated 
 

with 
 

drop 
 

in 
 

egg 
 

 
production in poultry. 
Among the two serological tests namely, HI and 
ELISA employed for the detection of EDS-76 viral 
antibody, HI was found to be simple, sensitive and 
reliable. It is concluded that HI test could be used 
for the detection of EDS-76 infection in poultry 
flocks. 
 

 
Restriction 
 

DNA fingerprinting 
 

of 
 

the 
 

three 
 

 
indigenous strains were carried out in conjunction with 
the reference strain to check for any genetic variation 
between the strains. Comparison of the DNA fingerprint 
 
of all the four strains digested with restriction 
 

 
endonucleases 
 

BamHI, 
 

EcoRI 
 

and 
 

HindIII 
 

revealed 
 

 
identical 
 

banding pattern 
 

thereby 
 

conforming 
 

the 
 

 
genetic similarity of the strains. 


UR  - http://krishikosh.egranth.ac.in/handle/1/5810104568
ER  -