TY  - BOOK
AU  - Lydia Priscilla K 
AU  - Balakrishnan P P(Guide)
TI  - Classification And Characterization Of Follicular Oocytes Of Crossbred Cattle
U1  - 636.082 
PY  - 2001///
CY  - Mannuthy 
PB  - Department of Animal Reproduction, College of Veterinary and Animal Sciences 
N2  - The objective of the present study was to analyse the 
ovarian factors that would influence the type of oocyte recovered for 
further use in terms of in vitro embryo production. The normal 
ovaries of crossbred cows collected randomly from the slaughter 
house were classified into four stages Stage I (SI), Stage Il (S2), 
Stage III (S3) and Stage IV (S4) of the oestrous cycle according to the 
visual appearance of CL and ovarian morphology. 
The length, width, thickness and weight of the ovary 
were significantly influenced by the stage of the oestrous cycle and 
the maximum value was recorded during the S3 stage (late luteal 
phase) of the cycle. The average values recorded irrespective of the 
stage for length, width, thickness and weight of the ovary was 
2.47±O.03cm, 1.81±O.03cm, 1.42±O.03cm and 4.05±O.14g respectively. 
The mean number of vesicular follicles belonging to the 
4 to 8mm category was significantly influenced by the stage of the 
oestrous cycle (p<O.05). But the number of <4mm and >8mm 
diameter follicles were not influenced by the stage of the oestrous 
cycle. The late luteal phase (S3) had more number of follicles in less 
than 4mm category whereas S3 and S4 stage had more number of 
follicles in 4 to 8mm and >8mm category. 
 

 
 

The size of the follicle had an interactive effect along 
with the quality of oocyte obtained in each category of the follicle. 
The number of oocytes obtained from <4mm sized follicle was 
significantly high (p<O.Ol) than the number observed in the other 
categories. The good quality oocytes were also found to be 
significantly higher in number when recovered from <4mm sized 
follicles. 
The recovery rate of oocytes as per the stage was found 
to have a statistical significance (p<O.Ol). There was a significantly 
higher output from ovaries belonging to the S3 phase than the. 
number obtained from SI, S2 and S4 stage. Moreover, the quality of 
the oocyte recovered from S3 stage was significantly influenced 
(p<O.Ol) than when compared to other stages which were 
statistically similar in their effect on oocyte quality. 
Slicing method yielded the maximum number of oocytes 
per ovary than when compared to the other techniques employed. 
There was significant difference in the recovery rate among the 
methods (p<O.Ol) with slicing yielding, high proportion of oocytes 
both in number and quality (6.27 and 61.53 per cent of grade I). The 
mean number of oocytes recovered by aspiration, puncturing and 
post aspiration slicing was 4.16, 3.67 and 3.13 respectively. The 
method of recovery and the quality of oocyte recovered had an 
 
interactive and significant effect on the oocyte recovery rate 
(p<O.Ol). 
The level of sodium, potassium, iron and copper in the 
follicles of various diameters present during the different stages of 
the cycle in the ovaries was found to vary significantly between 
stages. The level of zinc was not influenced by the stages of the cycle. 
The variation in the concentration of the minerals was found to be 
related with the normal physiological changes that occurred during 
the different stages of the cycle. 


UR  - http://krishikosh.egranth.ac.in/handle/1/5810105007
ER  -