TY  - BOOK
AU  - Murugan C 
AU  - Sujatha V S (Guide)
TI  - Molecular characterization of piper species usiing RAPD technique
U1  - 633.8 
PY  - 2002///
CY  - Vellanikkara
PB  - Department of Plantation Crops and Spices, College of Horticulture
N2  - The study on "Molecular characterization of Piper species using RAPD 
techniques" was conducted in the Department of Plantation Crops and Spices and 
CPBMB, College of Horticulture, Vellanikkara, Thrissur from December, 1999 to 
August, 2001. Material included 13 species/accessions of the genus Piper including 
Piper nigrutn Linn. These were analysed with 20 selected oligomer decamer primers 
using PCR technique and agarose gel electrophoresis. 
The selection of ideal DNA isolation technique was done based on 
observations of purity of bands, quality of the DNA bands and quantity of DNA 
recovered. The protocol of Edwards et al. (1991) was selected for DNA isolation 
with additional step of chloroform-isoamyl alcohol treatment. 
Fifty decamer primers were screened using DNA. of P. nigrum and 20 
were selected based on the number of bands produced, reproducibility of the bands 
and distinctness of the RAPD profile. 
RAPD profile of the 13 species of Piper were compared for genetic 
similarity index (using Jaccard's coefficient), with all 20 selected primers 
separately. Pooled similarity of the twenty primers put together was found out for 9 
species which gave amplification for all the 20 selected primers. The results of 
pooled analysis was subjected to cluster analysis (SAl-IN) and phenetic dcndrogram 
was constructed employing UPGMA. 
Four clusters were obtained, comprising of two accessions of P. nigrum 
In the first, two accessions of P. longum in the second, P. colubrinutn and 
P. attenuatum forming the third cluster and P. chaba, P. belle and P. arboreum 
forming the fourth. 


UR  - https://krishikosh.egranth.ac.in/handle/1/5810159753
ER  -