TY  - BOOK
AU  - Hazeena M S 
AU  - Sulekha G R (Guide)
TI  - Standardisation of in vitro techniques for the rapid clonal propagation of bael (Aegle marmelos (L) corr)
U1  - 633.8 
PY  - 2001///
CY  - Vellayani
PB  - Department of Plantation Crops and Spices, College of Agriculture
N2  - Studies were conducted for evolving in vitro techniques for the rapid 
clonal propagation of bael [ Aegle marmelos CL.) Corr.] during 1999 - 2001 at 
Plant Molecular Biology and Biotechnology Centre, College of Agriculture, 
Vellayani. 
Attempts were made to standardise in vitro propagation techniques of 
bael via enhanced release of axillary buds using shoot nodal segments and 
cotyledons as explants. Entire cotyledons were used as explants for direct 
organogenesis while cotyledons excluding embryoaxes were used for indirect 
organogenesis. 
Among the explants used for enhanced release of axillary buds, 
cotyledons responded better than nodal segments. Cent per cent survival could 
be obtained in cultures with cotyledons while a maximum of 50.00 per cent 
was obtained in cultures with nodal segments. 
Maximum shoot proliferation from nodal segments was obtained on 
full strength MS basal medium supplemented with BA 2.50 mgl ", IAA 1.00 
mgl ", sucrose 30.00 gr' and agar 8.00 gl'. 
The best treatment identified for shoot proliferation from cotyledons 
was full strength MS basal medium supplemented with BA 0.50 gl ", GA3 3.00 
mgl', adenine sulphate 20.00 mgl", sucrose 50.00 gl' and agar 5.00 gr'. 
Maximum initiation of direct organogenesis from cotyledons ( 83.33 
per cent ) occurred in two treatments namely, on full strength MS basal 
 
~--------------~~~.---------------------- 
medium supplemented with BA 0.10 mgl ", "sucrose 30.00 gr', and agar 8.00 
gr' and on the same basal medium with BA 0.20 mgl ", sucrose 30.00 gl " and 
agar 8.00 gr'. The best treatment identified for highest number of shoots per 
culture was full strength MS medium supplemented with BA 0.40 mgl ", 
sucrose 30.00 gr' and agar 8.00 gri. Maximum proliferation of shoots on 
further subculturing was obtained on full strength MS medium supplemented 
with BA 0.20 mgl", IAA 2.00 mgl ", sucrose 30.00 gr' and agar 8.00 gr'. 
Direct organogenesis from in vitro root could be best obtained on full strength 
MS medium supplemented with BA 0.50 rngl", sucrose 30.00 gr' and agar 
8.00 gr'. 
The best treatment identified for callus initiation was full strength MS 
medium supplemented with BA 0.50 mgl', 2,4-D 0.50 rngl ", sucrose 30.00 
gr' and agar 8.00 gr' which recorded the highest callus index (350.00). Ideal 
treatment for the maximum proliferation from callus via indirect somatic 
organogenesis was found to be full strength MS medium with BA 2.00 rngl ", 
IAA 0.50 mgl", sucrose 30.00 gr' and agar 8.00 gr'. 
In vitro rooting occurred at its best on full strength MS medium 
supplemented with IBA 2.50 mgl ", sucrose 20.00 mgl' and agar 8.00 gr'. 
Pre-treatment with IBA 1000.00 ppm for 20 seconds proved to be the best for 
ex vitro rooting. 
Sand was the ideal potting media for ex vitro establishment. 


UR  - http://krishikosh.egranth.ac.in/handle/1/5810105686
ER  -