TY - BOOK AU - Devanand c b AU - Muraleedharan Nayar K N (Guide) TI - Processed canine and Fish Collagen Sheets for cytoplasty in Rabits and Dogs U1 - 636.0897 PY - 2002/// CY - Mannuthy PB - Department of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences N2 - The study was conducted with the objective of evaluating the host acceptability and healing of processed collagen sheets of animal and aquatic tissue for reconstruction of experimentally created urinary bladder defects in rabbits and for reconstruction of urinary bladder in clinical cases in dogs. The study was conducted in twelve apparently healthy adult New Zealand White rabbits of either sex weighin~ 2.1 - 3 kg, divided into two groups, I and 11., of six animals each and in six male dogs (group Ill). The rabbits were subjected to partial cystectomy followed by cystoplasty using glutaraldehyde processed canine collagen sheet in group I, diacetyl processed fish collagen sheet in group 11 and in clinical cases in dogs, cystoplasty using both the materials. Tissue samples of urinary bladder harvested from died dogs were made into sheets, processed and crosslinked with glutaraldehyde at Central Leather Research Institute, Adayar, Chennai. Fish air bladder collected from larger fresh water fishes were made into sheets, processed and crosslinked with diacetyl at Central Institute of Fisheries Technology, Cochin. The collagen sheets were sterilised with gamma irradiation. The fish collagen sheet was thicker than canine collagen sheet. All the rabbits were premedicated with atropine sulphate and xylazine hydrochloride and were anaesthetized using ketamine hydrochloride. In the rabbits a portion of bladder wall (2 cnr') was removed from dorsal aspect of fundus close to the vertex. The graft material was fixed at the defects, using 5-0 braided silk in overlapping continuous horizontal mattress sutures. The collagen sheet effected haemostasis at the bleeding points of surgically created defects. • IJ The rabbits of both the groups were kept under observation for a minimum period of one month. The animals of both the groups were lethargic and dull on the first postoperative day and active and alert from second day. A marginal increase in rectal temperature, pulse rate and gradual decrease in respiration rate were observed during early postoperative period in all the animals. A reduction in feed and grass intake, and water consumption was noticed on the first postoperative day in all the animals and normal feeding and water i~take were resumed from second postoperative day. Marginal reduction in haeomoglobin concentration, packed cell volume, total erythrocyte count, and totalleukocyte count were observed on seventh postoperative day in haemogram. Differential leukocyte count showed neutrophilia in both the groups and decrease in lymphocyte count on seventh postoperative day. Eosinophil, basophil and monocyte counts showed marginal variations. During the early period of observation an increase in blood urea nitrogen was noticed in both the groups, but serum creatinine level did not show any variation. A marginal increase in serum sodium, potassium and decrease in chloride concentration, total protein and albumin were noticed during the period of observation in both the groups. Intravenous pyelogram during postoperative period showed normal passage of urine from renal level and normal filling of reconstructed bladder in all the animals. III Gross morphological changes were not observed externally on the bladder or on the mucosa. Moderate fibrinous adhesions were noticed between grafted site and adjacent tissues, but mild corded thickening of the mucosa bordering the central portion of grafted area was observed. Histomorphological studies by 30th day revealed almost complete proliferation of mucosa with progressive neovascularisation and inflammatory reaction in both the groups. The proliferation of blood vessels and inflammatory reactions were much greater in group 11. In clinical study in the dogs cystoplasty was done using canine and fish collagen sheets. All the dogs were premedicated with triflupromazine hydrochloride and anaesthetized using thiopentone sodium 'to effect'. The rent on the bladder was reconstructed by fixing the graft material to the edges of the bladder using 3-0 catgut in through and through continuous horizontal mattress sutures. The dogs were kept under observation for 15 days postoperatively. The animals started feeding on milk from first postoperative day and gradually attained normal appetite by fourth day. Animals passed blood tinged urine for two to three days postoperatively. Urine pH was slightly alkaline for first two days after surgery. The urine samples were positive for occult blood and protein for first two days. Microscopically urine sample revealed granular casts, phosphate crystals, epithelial cells, red blood cells and white blood cells during the observation period. Straining while urination was observed for the first day in all the dogs but it ceased from second day. , iv A marginal increase in rectal temperature, pulse rate and respiration rate were observed during early postoperative period in all the dogs. A marginal decrease in haemoglobin concentration and increase in packed cell volume, total erythrocyte count, totallymphocyte count were the salient features of haemogram during early postoperative period. Differential leukocyte count showed mild neutrophilia in all the dogs. A decrease in lymphocyte count was noticed during the early period of observation. The eosinophil, basophil and monocyte count showed marginal variation during the period of observation. During the early period of observation an increase in blood urea nitrogen with no variation in serum creatinine level were noticed in all the dogs. Marginal elevation in serum sodium and potassium concentration and marginal fall in chloride concentration during the early period of observation was noticed in all the dogs. A marginal decrease in total serum protein and increase in serum albumin was noticed in early period of observation. All the dogs regained near normalcy by 15th day postoperatively. Retrograde cystogram during the postoperative period revealed normal contour and moderate filling capacity of reconstructed bladder. UR - http://krishikosh.egranth.ac.in/handle/1/5810015033 ER -