TY  - BOOK
AU  - Suprabha P 
AU  - Anilkumar K (Guide)
TI  - Molecular genetics diversity in dwarf cattle of Kerala 
U1  - 636.082 
PY  - 2003///
CY  - Mannuthy
PB  - Department of Animal Breeding and Genetics, College of Veterinary and Animal Sciences
N2  - This study was undertaken for finding the molecular genetic diversity 
among dwarf cattle groups of Kerala namely Vechur, Highrange dwarf, Vatakara 
and Kasargode using RAPD-PCR technique. 
Genomic DNA was isolated from 101 animals belonging to the four cattle 
groups using phenol: chloroform method. The mean yields of DNA isolated from 
five ml of whole blood was 110.07 ± 9.4 ug and the average ratio between optical 
densities at 260 and 280 nm was 1.6. The PCR conditions were standardized 
with respect to annealing temperature (382c), magnesium chloride concentration 
(1.5 mM) and the amount of Taq DNA polymerase (0.5 units/20 Ill). RAPD-PCR 
analysis was performed in pooled DNA samples using 26 random 
oligonucleotides. Individual sample analysis was done by selecting ten primers. 
The degree of polymorphism obtained for the primers varied from 0 to 100 per 
cent in different populations. The polymorphism of primers ILO 876, ILO 1127, 
OPA 20, OPA 12, OPA 09 were higher for all groups tested. ILO 526, OPA 10, 
OPA 01 and OPA 18 gave moderate levels of polymorphism whereas the 
polymorphism given by OPA 06 was very low. 
The primer [LO 1127 produced a distinct band which was present in half 
of Vechur animals and in one Kasargode cattle. ILO 526 gave a low degree of 
polymorphism in Kasargode cattle and moderate levels in other groups tested. A 
predominant population specific band of 2.5 kb was identified in Highrange 
dwarf cattle with primer OPA 06. When amplified with OPA 20, a band 
(OPA 20 c) was noticed in Vechur animals with a frequency 0.07 and it was 
absent in other animals. The primer OPA 18 produced two common bands and 
the primer OPA 06 produced eight common bands. OPA 014, OPA 09 g and 
OPA 12 g were the common bands present in all the animals tested when 
respective primers were used. 
 
The band sharing values obtained were higher for Vechur and Vatakara 
animals (0.83 ± 0.00) and lower for Highrange dwarf and Kasargode animals 
(0.81 ± 0.00), when within group band sharing was considered. The between 
group band sharing value varied from 0.80 ± 0.00 (between Highrange dwarf and 
Kasargode) to 0.83 ± 0.00 (between Vechur and Vatakara). This indicates that 
Vechur and Vatakara populations are more similar than Highrange dwarf and 
Kasargode cattle. The least squares analysis of variance of band sharing value 
showed significant effects (P<O.O 1) of genetic group, gel and GC content of 
primer on band sharing. The percentage difference values calculated as a 
measure of genetic divergence between groups were not significantly different 
between Vechur and Vatakara and between Highrange dwarf and Vatakara. They 
differed significantly between comparisons of Vechur-Highrange dwarf, 
Vatakara-Kasargode, Vechur-Kasargode and Highrange dwarf-Kasargode 
combinations. The mean average percentage difference calculated as a measure 
of genetic dissimilarities between groups, ranged from 17.93 (between Vechur 
and Vatakara) to 20.92 (between Highrange dwarf and Kasargode). The genetic 
distance calculated was lowest between Vechur and Vatakara (0.006) and highest 
between Highrange dwarf and Kasargode (0.046). The dendrogram prepared 
showed mixing of animals of different genetic groups. 
In the present study the molecular genetic diversity among four genetic 
groups of dwarf cattle in Kerala was estimated using the RAPD-PCR technique. 
It was observed that the Vechur and the Vatakara animals are genetically more 
similar than the Highrange dwarf and Kasargode animals. 


UR  - https://krishikosh.egranth.ac.in/handle/1/5810106027
ER  -