TY  - BOOK
AU  - Parvathy Venugopal 
AU  - P.O.Nameer(Guide)
TI  - DNA Barcoding of the insetivorous bats of Parambikulam tiger reserve western ghats, Kerala
U1  - 634.9 
PY  - 2013///
CY  - Vellanikkara 
PB  - Department of Wild life Sciences , College of Forestry 
N2  - The study was carried out at Parambikulam Tiger Reserve during 2012- 
2013 with an objective of DNA barcoding the insectivorous bats to unravel the 
taxonomic ambiguity. The methods employed were the phenol-chloroform 
extraction (Sambrook et al., 1989) or the GeniPurelM Mammalian Genomic DNA 
Purification Kit (GeNei™). The sequences were compared with those registered 
in NeB! databank (blast.ncbi.nlm.nih.gov). The phylogeny reconstruction and the 
calculation of genetic distances were done using the MEGA 5.0 (Tamura et al., 
2011). 
In this study, three bat species such as Hipposideros speoris, Megederma 
spasma and Rhinolophus rouxii, were identified using the partial cytochonne b 
sequence from nine samples of individuals. But the species, Megaderma spasma 
and Hipposideros speoris showed a genetic distance between two percent and 
11 % while compared with the Genbank sequences of those species. Bradly and 
Baker (2001) considered that a genetic distance more than 10% or between two 
percent and 11% at cytochrome b is an indication of species level divergence or 
valid species. So the high sequence divergence in Megaderma spasma and 
Hipposideros speoris of present study with the same species from South Asia and 
South India gives an idea on the existence of conspecific population or valid 
species in these localities. The high sequence divergence may possible as they 
were collected from two different geographic locations and they result from 
geographic barriers separating genetic flow. To identify this complexity, the 
present study recommends additional study concerning specific status and for that 
more specimens should be collected from each geographical location. Because of 
this high genetic distance or sequence divergence between the sequences of 
Megaderma spasma and Hipposideros speoris of present study and that of 
retrieved from Genbank were clustered as sister clades in their respective 
phylogenetic neighbour-joining tree. The two sequences of Megaderma spasma, 
 
KAUNHM2012318 and KAUNHM2012320, and one sequence of Hipposideros 
speoris, KAUNHM2012314, were identified as a haplotypes. 
In the case of Rhinolophus rouxii sequences, the phylogenetic analysis put 
KAUNHM20114 and KAUNHM201185 together in one branch and they again 
clustered to the GenBank sequences of 80 kHz phonic type of Rhinolophus rouxii. 
The genetic distance also confirms this. But the third sequence of Rhinolophus 
rouxii from the present study, KAUNHM2012310, clustered with the sequences 
of 90 kHz phonic type of Rhinolophus rouxii and that showed a genetic distance 
of 9.5% when compared with two other Rhinolophus rouxii sequences of present 
study. So, the study clearly indicates the existence of sibling species of 
Rhinolophus rouxii and Rhinolophus indorouxii in the study area and this support 
the study ofChattopadhyay et al. (2012). 


UR  - http://krishikosh.egranth.ac.in/handle/1/5810110155
ER  -