000			02405nam a22001697a 4500
999			_c155272 _d155272
082			_a660.6 _bVIP/ID
100			_aVipin Mohan
245			_aIdentification of functional markers for thermosensitive genic male sterile rice (Oryza sativa L.)
260			_aVellayani _bDepartment of Plant Biotechnology, College of Agriculture _c2016
300			_a45 plants
502			_aMSc
520			_aThe project entitled “Identification of functional markers for thermosensitive genic male sterile rice (Oryza sativa L.)” was conducted in Department of Plant Physiology, College of Agriculture, Vellayani, Thriruvananthapuram, during 2015-2016. The main objective of the study was the identification of a functional molecular marker (SSR-Simple Sequence Repeats) to identify the unknown TGMS gene. The pollen donors for this particular study were the two red rice varieties, Uma and Jyothi. The TGMS line used for this study was EC720903 imported from IRRI, Philippines. The mapping population for this particular study was the F1 and F2 populations obtained from the crosses between Uma and Jyothi with TGMS line (EC720903). For obtaining the F1 population we adopted proximal hybridization method. The F1 plants were allowed to self-pollinate for getting F2 population. In this present study, twenty nine SSR primers have been used for initial screening with parents. Out of twenty nine SSR primers only three showed polymorphism viz; RM5897, RM257 and RM3351 in 3.5% agarose gel. One of these polymorphic markers showed polymorphism in urea PAGE. That marker was RM5897 with product size 141bp, which was associated with tms5 gene with a distance of 1.45 cM and located on chromosome number 2. The data obtained from urea PAGE analysis was in correlation with the phenotypic data obtained from anther staining of F2 plants at flowering stage with 1% Iodo potassium iodide. The present study revealed that the gene conferring male sterlity present in this TGMS line is tms5 and which is located in chromosome number 2. The identified marker RM5897 associated with tms5 gene is functional for selecting progenies for further backcross breeding programmes (so that identification of male sterile plants in seedling stage itself will be possible).
650			_aPlant Biotechnology
700			_aRoy Stephen (Guide)
856			_uhttp://krishikosh.egranth.ac.in/handle/1/5810025078
942			_2ddc _cTH