000			02528nam a2200181Ia 4500
999			_c25918 _d25918
003			OSt
005			20220823140113.0
008			140128s9999 xx 000 0 und d
082			_a633.8 _bAMB/CY
100			_aAmbili Anand
245			_aCyto-Morphological Investigations in Piper Spp.
260			_aVellanikkara _bDepartment of Plantation Crops and Spices, College of Horticulture _c1997
502			_bMSc
520	3		_aThe study 'Cyto-morphological investigations in Piper spp.' was conducted in the Department of Plantation Crops and Spices, College of Horticulture, Vellanikkara during the period from October 1994 to December 1996. The major objectives were to prepare morphological descriptors for the eight Piper spp. collected from different geographical areas and to conduct cytological investigations in these species. To prepare morphological descriptors for the eight species studied detailed morphological observations were recorded. Based on the salient observations on morphology brief descriptions of the eight Piper spp. were made. The eight species of Piper studied were analysed for the similarities and differences in their morphological characters to find out the relationship among the species. Based on morphological comparisons, a key for identification of species has been proposed. The procedure for mitotic studies in Piper spp. was standardised. The roots were collected between 11.15 a.m. and 12.15 p.m. and pre-treated in 8- hydroxyquinoline for two to three hours at 40 C. The pre-treated roots were fixed in Carnoy's A or Carnoy's B for one or two days. The material was then hydrolysed in 1 N hydrochloric acid in a water bath maintained at a temperature of 600C for fifteen minutes. The roots were then stained in 0.5% acetoorcein for 45 minutes. After that slide preparations were made and examined for the presence of mitotic chromosomes. Microphotographs were taken and good slides were made permanent. Chromosome number of 32, 52, 32, 32, 24, 26, 32, 52 and 52 were observed in P. argyrophyllum, P. attenuatum, P. bababudani, P. betle, P. chaba, P. colubrinum, P. longum, P. nigrum (Panniyur-I) and P. nigrum (wild) respectively: Except for the South American species, P. colubrinum, all the species studied possessed chromosomes in multiples of four suggesting a basic number of four for the Indian Piper.
700			_aSujatha V S (Guide)
856			_uhttps://krishikosh.egranth.ac.in/handle/1/5810155377
942			_2ddc _cTH