000			02162nam a2200181Ia 4500
999			_c26533 _d26533
003			OSt
005			20220827105737.0
008			140128s9999 xx 000 0 und d
082			_a639.2 _bBOM/ST
100			_aBomy Chummar
245			_aStudies on the Electrophoretic Pattern of Fish/ Shellfish Proteins Subjected to Frozen Storage
260			_aPanangad _bDepartment Of Processing Technology,College Of Fisheries _c2000
502			_bMFSc
520	3		_aFreezing ~ considered as an excellent process for preserving the quality of fish for longer periods. But freezing may affect various flesh components, especially the proteins. Sarcoplasmic proteins play a major role in species identification of fish and fishery products. While myofibrillar proteins play a major role in the jellying properties of surimi and surimi based products. The changes to these groups of proteins due to freezing and frozen storage have received much attention. Four species Megalaspis cordyla, Labeo rohita, Penaeus indicus and Parapenaeopsis styli/era were used for the frozen storage study. Samples were stored for 90 days at -18°C and samples were collected at 15 day. intervals and Salt Soluble Nitrogen (SSN), Water Soluble Nitrogen (WSN) and Non-Protein Nitrogen (NPN) content were determined. Electrophoretic pattern of both Salt Soluble Proteins (SSP) and Water Soluble Proteins (WSP) of frozen stored sample were compared with that of the fresh sample. SSN, WSN and NPN content showed significant decrease during 90 days frozen storage period. However changes in the WSN content of P.stylifera showed no significant difference. Electrophoretic pattern of WSP remained almost the same throughout the frozen storage period with some minor bands becoming less intense with storage period. Electrophoretic pattern of SSP showed a major change in the myosin. Heavy chain which became narrower and less intense with the concomitant appearance of high molecular weight protein aggregate at the top of the gel.
700			_aNambudiri D D (Guide)
856			_uhttp://krishikosh.egranth.ac.in/handle/1/5810144168
942			_2ddc _cTH