000			03459nam a2200181Ia 4500
999			_c26863 _d26863
003			OSt
005			20220830161453.0
008			140128s9999 xx 000 0 und d
082			_a633.8 _bHAZ/ST
100			_aHazeena M S
245			_aStandardisation of in vitro techniques for the rapid clonal propagation of bael (Aegle marmelos (L) corr)
260			_aVellayani _bDepartment of Plantation Crops and Spices, College of Agriculture _c2001
502			_bMSc
520	3		_aStudies were conducted for evolving in vitro techniques for the rapid clonal propagation of bael [ Aegle marmelos CL.) Corr.] during 1999 - 2001 at Plant Molecular Biology and Biotechnology Centre, College of Agriculture, Vellayani. Attempts were made to standardise in vitro propagation techniques of bael via enhanced release of axillary buds using shoot nodal segments and cotyledons as explants. Entire cotyledons were used as explants for direct organogenesis while cotyledons excluding embryoaxes were used for indirect organogenesis. Among the explants used for enhanced release of axillary buds, cotyledons responded better than nodal segments. Cent per cent survival could be obtained in cultures with cotyledons while a maximum of 50.00 per cent was obtained in cultures with nodal segments. Maximum shoot proliferation from nodal segments was obtained on full strength MS basal medium supplemented with BA 2.50 mgl ", IAA 1.00 mgl ", sucrose 30.00 gr' and agar 8.00 gl'. The best treatment identified for shoot proliferation from cotyledons was full strength MS basal medium supplemented with BA 0.50 gl ", GA3 3.00 mgl', adenine sulphate 20.00 mgl", sucrose 50.00 gl' and agar 5.00 gr'. Maximum initiation of direct organogenesis from cotyledons ( 83.33 per cent ) occurred in two treatments namely, on full strength MS basal ~--------------~~~.---------------------- medium supplemented with BA 0.10 mgl ", "sucrose 30.00 gr', and agar 8.00 gr' and on the same basal medium with BA 0.20 mgl ", sucrose 30.00 gl " and agar 8.00 gr'. The best treatment identified for highest number of shoots per culture was full strength MS medium supplemented with BA 0.40 mgl ", sucrose 30.00 gr' and agar 8.00 gri. Maximum proliferation of shoots on further subculturing was obtained on full strength MS medium supplemented with BA 0.20 mgl", IAA 2.00 mgl ", sucrose 30.00 gr' and agar 8.00 gr'. Direct organogenesis from in vitro root could be best obtained on full strength MS medium supplemented with BA 0.50 rngl", sucrose 30.00 gr' and agar 8.00 gr'. The best treatment identified for callus initiation was full strength MS medium supplemented with BA 0.50 mgl', 2,4-D 0.50 rngl ", sucrose 30.00 gr' and agar 8.00 gr' which recorded the highest callus index (350.00). Ideal treatment for the maximum proliferation from callus via indirect somatic organogenesis was found to be full strength MS medium with BA 2.00 rngl ", IAA 0.50 mgl", sucrose 30.00 gr' and agar 8.00 gr'. In vitro rooting occurred at its best on full strength MS medium supplemented with IBA 2.50 mgl ", sucrose 20.00 mgl' and agar 8.00 gr'. Pre-treatment with IBA 1000.00 ppm for 20 seconds proved to be the best for ex vitro rooting. Sand was the ideal potting media for ex vitro establishment.
700			_aSulekha G R (Guide)
856			_uhttp://krishikosh.egranth.ac.in/handle/1/5810105686
942			_2ddc _cTH