000			03478nam a2200181Ia 4500
999			_c27014 _d27014
003			OSt
005			20220901154050.0
008			140128s9999 xx 000 0 und d
082			_a633.8 _bGAN/MO
100			_aGanapathy V
245			_aMorphological And Phytochemical Investigations On St.John`s Wort (Hypericum Spp.), a Potential Source Of Ant-HIV Compounds
260			_aVellanikkara _bDepartment of Plantation Crops and Spices, College of Horticulture _c2003
502			_bMSc
520	3		_aRenowned laboratories throughout the world have been makuu; uucnse search hi detect and isolate potential chemicals from plants for curing AIDS. llvpcrtcmn is :\11 important genera yielding hypericin,a potential anti -I-IIV chemical I lvpcricin ll:l~ . been reported from many exotic Hypericum species. Investigations on the presence \Ii' hypericin in the indigenous flora (H mysorense and H jU/JOl1iClIl1I) arc i:ldiJl~' I kill" the present study entitled "Morphological and Phytochemical IJl\CSII!':lllllll~ .'11 St.Johns Wort (Hypericum spp.), a potential source or anti-HI V compounds" \I;I~ constituted. Two species of Hypericum were located in Kerala; H mysorcnsc .u the high ranges of Idukki and Wayanad districts and H. japonicum in Wayanad district. The natural habitat characters of H mysorense were studied at Vattavada in IduUI Phytosociological parameters such as density, abundance and per cent frequency were determined by quatrat studies. H mysorense was often present or seldom present , along with Pteridium aquilinum, Eucalyptus grandis, Lantana camara, Strobilanthus spp, Andropogon lividis and Chrysopogon zeylanicus in the high altitucle regions of Vattavada panchayat of Idukki district Chrysopogon zeylanicus is the dominant species in the eco system. H. mysorense is a shrub with stiff branches while H japonicum is a prostrate herb growing in marshy lands. Translucent glands were either streaks or dots in H. mysorense while it was pellucid dots in H japonicum. Stem anatomy of H mysorense revealed presence of "type B" secretory canals whereas it was absent in H japonicum. Histochemical tests indicated the presence of lignin in the leaf,stem and root of H. mysorense and H. japonicum. Leaf, stem and root of H. japonicum had phenolic compounds while there was no indication for the presence of these compounds in the stem of H. mysorense. The primary metabolites like starch, total sugars,protein and total free amino acids on leaf, stem and root were quantified. Qualitative test for anthraquinone was negative in both the species. When tested using TLC for the presence of hypericin ,no spots corresponding to In \ .iluc o ! standard hypericin were observed in H. mysorense and H. japonicum samples Commercial extract of H. perforatum (Eleve®,) had 0.22 to 0 25 per cell! hypericin. Even though the samples of the indigenous Hypericum sP/' (//. mysorense and H. japonicum ) were negative for hypericin - the target anti-l ll V compound, they were indicative for the presence of anthronoid derivati ves. These anthronoid derivatives could be the precursors or intermediary compounds for the hypericin synthesis in the plant metabolism. These compounds need further analysis for identification and characterization for anti-HfV property.
700			_aMini Raj N (Guide)
856			_uhttps://krishikosh.egranth.ac.in/handle/1/5810154986
942			_2ddc _cTH