000			02228nam a2200193Ia 4500
999			_c27794 _d27794
003			OSt
005			20220322153711.0
008			140128s9999 xx 000 0 und d
082			_a636.089 6 _bANU/DE
100			_aAnupama K
245			_aDetection of infectious bovine rhinotracheitis virus by immunofluorescence and polymerase chain reaction
260			_aMannuthy _bDepartment of Veterinary Microbiology, College of Veterinary and Animal Sciences _c2008
300			_a72
502			_bMVSc
520	3		_aA study was undertaken to detect the presence of IBR virus in clinical samples by PCR and immunofluorescence and to compare the efficacy of these tests. Attempts were also made to isolate the virus from clinical samples in MDBK cell line. A total of 60 samples from suspected animals and 5 samples from healthy animals were collected from various sources like, University Livestock Farms, Veterinary Hospital, Mannuthy and some private dairy farms in Thrissur district. The samples included nasal and vaginal swabs, aborted materials and semen. All these samples were screened for the presence of IBR virus by PCR and immunofluorescence. The TK gene based primers were used for PCR and the positive control generated an amplicon of size 298bp. No sample was found positive by PCR. Immunofluorescence was performed on smears prepared from clinical samples using FITC conjugated polyclonal antibovine IgG. This test detected viral antigen in one of the samples, indicated by focal, bright yellowish green fluorescence associated with the cytoplasm of epithelial cells. The inability of PCR for detection of viral DNA in sample tested positive by immunofluorescence may be due to the presence of non-specific inhibitors in clinical sample. These results suggest that even though PCR is highly sensitive compared to other diagnostic tests, false negative results cannot be excluded. Immunofluorescence on smears prepared from clinical samples can be considered as a rapid diagnostic method for IBR. Attempts to isolate the virus from clinical samples in MDBK cell line were found unsuccessful.
700			_aKoshy John(Guide)
856			_uhttp://krishikosh.egranth.ac.in/handle/1/5810046986
942			_2ddc _cTH