000			03847nam a22001817a 4500
999			_c289320 _d289320
082			_a630.28 _bARY/DE PG
100			_aArya S Nair
245			_aDevelopment of dual purpose fodder Cowpea [Vigna unguiculata (L.) Walp] through mutagenesis
260			_aVellayani _bDepartment of Plant Breeding and Genetics, College of Agriculture _c2021
300			_a97p.
502			_aM Sc
520			_aThe present study entitled “Development of dual purpose fodder cowpea [Vigna unguiculata (L.) Walp] through mutagenesis” was conducted in the Department of Plant Breeding and Genetics, College of Agriculture, Vellayani during 2019-2021. The study was conducted to induce mutation in fodder cowpea, variety Aiswarya with Ethyl Methane Sulphonate and to select superior dual purpose genotypes for seed and fodder from the mutants. The study was carried out in three experiments. In the first experiment, seeds of fodder cowpea variety Aiswarya were treated with five different doses of EMS. Observations were recorded on seed germination per cent and seedling survival per cent. Germination percentage and seedling survival percentage reduced with an increase in the concentration of the mutagen. Based on the seedling survival percentage, LD50 value were determined. Effective doses were fixed as 0.35%, 0.39%, 0.43%, 0.47% and 0.51% according to the LD50 value. In experiment II, seeds were treated with the effective doses of EMS and M1 plants were evaluated. Observations were recorded on seedling emergence (%), seedling survival at branching stage (%), and plant survival up to maturity (%) and these characters varied significantly among the treatments. Presence of chimera and chlorophyll mutants was also observed. Presence of chlorophyll mutants were noted in the population of all mutagenised treatments. Seeds from M1 generation constituted M2 population. M2 generation was observed for various quantitative characters including number of primary branches per plant, number of leaves per plant, days to first flowering, days to 50% flowering, green fodder yield per plant, dry matter yield per plant, leaf stem ratio, leaf dry weight per plant, stem dry weight per plant, leaf area index, plant height, seed yield per plant and days to maturity and qualitative characters like early plant vigour, plant growth habit, leaf texture, leaf colour, flower colour, seed colour and presence of chlorophyll mutants. Significant variations were found in all quantitative characters except plant height and leaf stem ratio. Among these, number of primary branches per plant, number of leaves per plant, green fodder yield per plant, dry matter yield per plant, leaf dry weight per plant, stem dry weight per plant and leaf area index were higher in 0.43% EMS treatment. Seed yield was found higher in control, which was on par with 0.51% EMS and followed by 0.43% EMS treatment. In qualitative characters, seed colour and plant growth habit showed remarkable variation, whereas early plant vigour, leaf texture and leaf colour exhibited slight variations. Flower colour did not show any variations. Comparing with M1, frequency of chlorophyll mutants in M2 was lesser. For fodder purpose, 0.43% EMS treatment expressed higher fodder yield. In case of seed yield, 0.51% was recorded with higher seed yield, followed by 0.43% among the mutants. It was concluded from the present investigation that, for fodder cowpea variety Aiswarya, which was treated with different concentration of EMS and 0.43% EMS treatment was found effective to obtain superior genotypes for seed and fodder yield from mutants.
650			_aPlant Breeding and Genetics
650			_aCowpea
650			_aVigna unguiculata (L.) Walp
700			_aGayathri, G (Guide)
942			_2ddc _cTH