Screening and characterization of rice genotypes for abiotic stress to develop climate resilient variety

Abstract

The study entitled as “Screening and characterization of rice genotypes for abiotic stress to develop climate resilient variety” was completed in Regional Agriculture Research Station, Pattambi, Palakkad during the period September 2019 to September 2020. 12 rice genotypes were screened morphologically using hydroponics. M-4, JS-1, Pokkali, Akshaya and N22 were selected as drought tolerant ones whereas M-4 and JS-1 alone were selected as saline tolerant ones. N22, M-9, JS-1, NPT culture-1 and Akshaya showed tolerance against high temperature in Temperature Induction Response reaction. Drought specific primers such as RM316, RM511 and RM302 were used for the charactrisation. JS-1 showed amplification for all the three primers whereas Kalluruli, NPT culture-1, Vyttila-9 Jyothsana and Manurathna had specific amplicons for two of the primers. Salinity specific primers used were RM493, RM3412 and RM10793. The variety Pokkali confirmed its tolerance against higher salt concentration by amplifying in all the three primers used. M-4 and JS-1 got amplified for two of the primers used. RM7076, RM5749 and RM26212 used as the screening sieve for high temperature tolerant varieties. N-22, being the universal check got amplified in all the three primers. JS-1 and Akshaya had amplicons for two of the primers which support the result of phenotyping. From the whole study, JS-1 was selected as the most promising genotype for multiple abiotic stress considering the performance in both phenotyping and genotyping. NPT culture-1 was selected as the second best performer in this study.