Physico-chemical characterization of gum-oleoresin from Ailanthus triphysa(Dennst.) Alston and effect of ethephon on gum-oleoresin yield.

Abstract

Gum and resins are natural bio-polymers having number of applications in pharmaceutical and food industries. Most of them are regarded as bio-degradable and safe because of their bio-compatibility, low cost, non-toxic, processing, environmental friendly and local availability. It is viable income source for thousands of forest dwellers. So the present study entitled “Physico-chemical characterization of gum-oleoresin from Ailanthus triphysa (Dennst) Alston and effect of ethephon on gum-oleoresin yield” was carried out at Arboretum, College of Forestry, Vellanikkara during the year 2018-2019. The main objective of the study was to assess the effect of different concentration of ethephon on gumoleoresin production in Ailanthus triphysa and also study the effect of diameter classes on production. The physical properties such as Solubility, pH, Viscosity, Moisture level, Color parameters, Ash content and chemical compounds were also determined as part of the study. A total of twenty-four trees were selected for conducting the study in which two girth classes (70-110cm, 111-150cm), four chemical treatments, each with three replications were taken. Data analysis was done on monthly intervals, showed significant difference in girth class on resin production in the months of June, July, August, November, December and April and in other months it found non-significant. It was observed that girth class G2 (111cm-150 cm) showed more yield compared to G1 (70-110). It was statistically found significant difference in gum-oleoresin yield due to different concentration of ethephon treatments. T4 (3.9%) ethephon treatment gave more yield (817.99gm) compared to other treatments, where T1 (control) gave very less yield (226.16gm). The exudation of gum-oleoresin was found to be more in post monsoon (55.06gm) followed by monsoon (43.78gm) and least during summer (27.01gm). Interaction between different ethephon treatment and season was found to be non-significant. 116 The physical and chemical properties of gum-oleoresin were studied and it indicated the gum-oleoresin was found to be strongly acidic, highly soluble in xylene and least in chloroform and in case of water, it absorbs water and swell. Moisture content was 5.17%, pH observed was 5.5, Colour of the gum-oleoresin observed was pale yellow to dark reddish brown and Viscosity observed was 2340 cps and 695 cps. Heavy metals like K ˃ Mg ˃ (Ca) ˃ (Ir) were detected. Chemical compounds were identified by using GC-MS. Total 18 compounds were identified from Acetone extract of gum-oleoresin in GC-MS, the major constituents of the chemical compound were beta.-caryophyllen followed by Tetradecane and 1,3,3-trimethyl-2-(2-methylcyclopropyl)-1-cyclohexene and 1- octadecene constitute least contribution, where as in methanol extracts, 16 compounds were identified, most abundant compound was Caryophyllene followed by 1,2-benzenedicarboxylic acid and bicyclo, the least abundant constituent was found to be 3-hexadecene. The correlation studies of bark thickness on production of gum-oleoresin were found to be significant with correlation coefficient 0.42. The climatic parameters like rainfall, relative humidity, temperature was found to be non-significant on production of gum-oleoresin.