Bio-efficacy of tank mixed herbicides and urea in wet seeded rice

Abstract

The use of herbicides has become an indispensable part of agriculture, particularly in rice cultivation, where weeds are the most harmful of the biotic constraints to production. A usual practice among rice farmers in the Kole lands of Kerala is the tank mixing of herbicides, for a broader spectrum of weed control, enhanced weed control efficiency and reduction in the cost of application. However, mixing of herbicides is done without any knowledge of the probable synergistic or antagonistic interactions on weed flora. Herbicides are also mixed with fertilizers like urea under the common belief that herbicidal efficiency is enhanced by this practice. Application of herbicides may affect various plant pathogens and microbial bioagents which occur naturally in the rice ecosystem. Information of the effect on main disease causing as well as beneficial microorganisms would help in selecting appropriate herbicides. Hence the major objectives of the research programme entitled “Bio-efficacy of tank mixed herbicides and urea in wet seeded rice” were to study the synergistic or antagonistic effect of tank mixing of commonly used herbicides, and to evaluate the efficiency of these herbicides on mixing with urea, on major weeds of rice. The effect of these herbicides on beneficial and pathogenic microorganisms were also investigated. Two field experiments were conducted at Alappad padasekharam in the Kole lands of Thrissur from October to January 2019-20 and 2020-21. The soil of experimental field was clayey in texture, high in organic carbon (1.1-1.3%), low available nitrogen (180-188 kg/ha), and medium phosphorus (20-21.5 kg/ha) and potash (152-159 kg/ha), with a soil pH of 4.5-4.7. The feasibility of mixing herbicides was evaluated in experiment I (Bio-efficacy of tank mixed herbicide combinations in wet seeded rice). There were fourteen treatments and they included tank mixtures and sequential applications of five herbicides viz., fenoxaprop-p-ethyl (0.06 kg/ha), (chlorimuron-ethyl + metsulfuron-ethyl) (0.004 kg/ha), carfentrazone ethyl (0.02 ii kg/ha), bispyribac-sodium (0.025 kg/ha), and (cyhalofop-butyl + penoxsulam) (0.15 kg/ha). These were compared with two broad spectrum herbicides, bispyribac-sodium (0.025 kg/ha) and (cyhalofop-butyl + penoxsulam) (0.15 kg/ha), as well hand weeded and unweeded controls. In experiment II (Bio-efficacy of tank mixed herbicides and urea in wet seeded rice), there were twelve treatments. Six herbicides, viz., cyhalofop butyl (0.08 kg/ha), (cyhalofop butyl + penoxsulam) (0.15 kg/ha), bispyribac-sodium (0.025 kg/ha), fenoxaprop-p-ethyl (0.06 kg/ha), carfentrazone ethyl (0.02 kg/ha) and (chlorimuron ethyl + metsulfuron methyl) (0.004 kg/ha) were applied with and without urea (1%) mixing. Hand weeded and unweeded controls were also included for comparison. Randomized Block Design with three replications was adopted for both experiments. Wet seeding of rice at the seed rate of 100 kg/ha was done. Nitrogen, phosphorus and potassium @ 90:35:45 kg/ha were supplied through urea, factomphos and muriate of potash (KAU, 2016). In experiment III (In vitro evaluation of herbicides on beneficial and pathogenic microorganisms) treatments included the effect of herbicides mentioned in experiment II which were evaluated under in vitro conditions with recommended, higher and lower doses on beneficial microbial bioagents (Trichoderma viride and Pseudomonas fluorescens) and pathogenic microorganisms (Rhizoctonia solani, Pyricularia oryzae and Xanthomonas oryzae pv. oryzae). Factorial Completely Randomized Block Design was adopted with 18 treatments and three replications each. Tank mixing of herbicides was found to be more effective than their sequential application for most of the combinations. Tank mixed combination of (cyhalofopbutyl + penoxsulam) with (chlorimuron-ethyl + metsulfuron-methyl) showed synergistic effect and had lowest weed density (32, 16 and 20 nos./m2 ), lowest weed dry matter production (14.68, 50.50 and 88.95 kg/ha), and highest weed control efficiency (91, 88, and 82%) at 15, 30 and 60 days after application respectively. The number of tillers per sq. m (411), number of panicles per sq. m (288), number of grains per panicle (103), percentage of filled grain (91.6) at harvest, nutrient uptake by rice at 60 DAS (105.06, 14.32 and 74.28 kg/ha of N, P and K respectively), grain iii yield (3.97 t/ha), net returns (Rs.71,406/ha) and benefit: cost ratio (2.25) were highest in the same treatment. The next best treatment was fenoxaprop-p-ethyl + bispyribac sodium. On tank mixing of herbicides with urea, bispyribac-sodium and urea interacted synergistically, resulting in highest weed control efficiency (82.5 and 78% at 15 and 30 days after application respectively), highest grain yield (5.03 t/ha), and profitability (Rs. 93,509/ha) over the other treatments. Mixing of (cyhalofop-butyl + penoxsulam) with urea could also be recommended for good results (grain yield of 4.94 t/ha and net returns of Rs.87,463/ha). In vitro evaluation of herbicides revealed that bispyribac-sodium and (cyhalofop-butyl + penoxsulam) had greatest inhibitory effect on pathogenic microorganisms Rhizoctonia solani and Pyricularia oryzae, but showed no effect on Xanthomonas oryzae pv. oryzae, and were less harmful to biocontrol agents Trichoderma viride and Pseudomonas fluorescens at recommended doses. Tank mix combination of (cyhalofop-butyl + penoxsulam) with (chlorimuronethyl + metsulfuron-methyl) was the best treatment with compatibility, efficient weed control, high grain yield and net returns. Tank mixing of bispyribac-sodium with urea 1% had synergistic effect resulting in highest weed control efficiency, production and profitability over the other treatments. Mixing of (cyhalofop-butyl + penoxsulam) with urea 1% could also be recommended for good results. Bispyribac-sodium and (cyhalofop-butyl + penoxsulam) were the best herbicides on in vitro evaluation in their effect on pathogenic microorganisms Rhizoctonia solani and Pyricularia oryzae, and at recommended doses were compatible with biocontrol agents Trichoderma viride and Pseudomonas flourescens.