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  1. Kerala Agricultural University Digital Library
  2. 1. KAUTIR (Kerala Agricultural University Theses Information and Retrieval)
  3. PG Thesis
a
Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/6157
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dc.contributor.advisorRajendran, P C-
dc.contributor.authorAbdulla Fayas, T-
dc.date.accessioned2019-10-22T05:49:45Z-
dc.date.available2019-10-22T05:49:45Z-
dc.date.issued2008-
dc.identifier.citationCoh T-1310en_US
dc.identifier.urihttp://hdl.handle.net/123456789/6157-
dc.description.abstractGenerating renewable source of energy from tissue culture laboratory waste by the process of biomethanogenesis is the focal theme of present investigation. Unlike developed countries, the developing countries are hesitant to establish more number of biotechnology/ tissue culture laboratories due to financial constraints. Easy and regular availability of biogas from TC wastes will be a boon to establish self-sustainable TC laboratory in view of present energy crisis. The biogas experimental units required for the study was designed and various treatments were employed for the biodegradation of tissue culture waste, using the methanogenic bacteria Methanobacterium ruminatium, Methanobacterium formicicum, Methcmosarcina barkeri, Bactereoides ruminicola, Selenomonas ruminatium, Eubacterium tortuosum and Clostridium butyricum. Treatment involving TC waste and cow dung was also conducted for biomethanation in the present study. Quantity of gas production and its combustibility was noticed for various treatments. In bacterial treatments the quantity of gas generation was highest for Clostridium butyricum. Only treatments involving cow dung produced combustible gas. Molecular characterization of methanogenic bacterial cultures was also done for finding the genetic similarity between them. RAPD followed by scoring of the bands by UPGA routine showed maximum similarity between bacterial cultures of Methanobacterium ruminatium and Methanobacterium formicicum with Methanosarcina barkeri. Physio-chemical characters like C/N ratio of the TC wastes, pH and temperature of medium and Hydraulic retention time was also observed for the various treatments. The C/N ratio of the TC wastes was found to be very low and nowhere near the optimum C/N ratio of 20-30 required for gas production. Other parameters like pH of the treatments and Hydraulic retention time was also noticed. The pH of the treatments involving bacterial cultures was very low, considering the normal pH of 6.8 to 7.5 required in biogas generation. The main constraints in the bio gas generation were found out to be the low C/N ratio of the TC waste and the low pH of the medium. The present study indicated the possibility of bio-gas generation from TC waste through fortification using various supplements like coconut water and coir pith which have higher C/N ratio.en_US
dc.language.isoenen_US
dc.publisherCentre for Plant Biotechnology and Molecular Biology, College of Horticulture, Vellanikaraen_US
dc.subjectBiogasen_US
dc.subjectBiogas generationen_US
dc.subjectMethanogenesisen_US
dc.subjectBiochemical features of methanogenic bacteriaen_US
dc.subjectCulture technique for anaerobesen_US
dc.subjectBiogas experimental systemen_US
dc.subjectQuantity of tissue culture waste availabilityen_US
dc.subjectIsolation of methanogensen_US
dc.titleManagement of biodegradable plant tissue culture lab wastes through biomethanogenesisen_US
dc.typeThesisen_US
Appears in Collections:PG Thesis

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