Effect of agrochemicals on biological activity in lateritic soil

Abstract

In modern agriculture, agrochemicals are frequently used to boost crop production. The use of pesticides is indispensable in various crops and cropping systems, particularly under tropical climatic condition. However erratic application of these chemicals may affect soil health, which in turn change the population and composition of soil microflora and soil fertility. The land use type also play a significant role in controlling the microbial community. Hence, the present study was undertaken at College of Horticulture, Vellanikkara during 2017-2018 so as to determine the impact of three agrochemicals viz. oxyfluorfen, copper oxychloride and chlorpyriphos on the population of soil microflora and dehydrogenase enzyme activity in lateritic soil collected from two land use types. Representative soil samples were collected from two land use types viz., land under cultivated grass, Agronomy farm, College of Horticulture, Vellanikkara (LUT 1) and cocoa plantation, Cocoa Research Center, KAU, Vellanikkara (LUT 2) during August, 2017. The soil samples were analyzed to assess physical, chemical and biological characteristics before starting experiment and the data revealed that soil collected from LUT 2 was more conducive for microbial growth and dehydrogenase activity. Pot culture experiment with cowpea (variety: Bhagyalakshmi) was conducted during March to June, 2018 with fourteen treatments (3 agrochemicals at 2 different doses under each land use types + 2 controls, one each for two land use types) and three replications. Agrochemicals viz., oxyflourfen (OXY) at 0.15 and 0.30 kg ha-1 (herbicide), copper oxychloride (COC) at 0.75 and 1.50 kg ha-1 (fungicide) and chlorpyriphos (CHL) at 0.30 and 0.60 kg ha-1 (insecticide) were selected for the study. Soil samples were analyzed at four intervals viz., 7, 30, 60 and 90 days after agrochemical spraying (DAS) to evaluate the effect of agrochemicals on chemical and biological characteristics of soil. The chemical characteristics of soil viz., pH, electrical conductivity, organic carbon, available N, P, K, Ca, Mg, S and micro nutrients like Fe, Mn, Cu, and Zn followed a decreasing trend towards the harvest in all the treatments under both the land use types. The effect of agrochemical application on chemical characteristics and nutrient availability of the soil was at lower magnitude compared to their influence on biological activity of the soil. The biological characteristics of soil viz. dehydrogenase activity and microbial population (fungi, bacteria and actinomycetes) were higher in soil samples collected from LUT 2 (cocoa plantation) at all the sampling intervals. Soil samples from both the land use types registered reduction in biological activity due to agrochemical application, which was higher at 7 DAS than the other sampling intervals. The extent of reduction in biological activity after application of agrochemicals was comparatively higher in LUT 1 (land under cultivated grass). Higher doses of the pesticides showed greater decline compared to their lower doses. Biological activity increased from 7 to 60 DAS and the extent of reduction with respect to control also became lower. At 90 DAS, a declining trend in the dehydrogenase activity and microbial population (except fungi) was noticed. The adverse effect of agrochemicals on dehydrogenase activity and bacterial population at 7 DAS followed the order: CHL > COC > OXY and OXY > CHL > COC respectively. However at 90 DAS, oxyflourfen exerted minimum adverse effect on soil bacteria. The study revealed that dehydrogenase activity is highly correlated with the population of soil microflora, especially soil bacteria. The adverse effect of agrochemicals on fungal population can be ranked as: COC > OXY > CHL at both 7 and 90 DAS. The percentage inhibition of actinomycetes population at 7 as well as 90 days after agrochemical application was in the order: OXY > COC > CHL. Cowpea yield was not affected by the agrochemical application and land use types. However higher doses of all the agrochemicals under study reduced the number of root nodules in cowpea.