Detection of Ralstonia solanacearum race 3 causing bacterial wilt of solanaceous vegetables in Kerala, using random amplified polymorphic DNA (RAPD) analysis

Deepa, James; Girija, D; Sally K, Mathew; Nazeem, P A; Babu, T D; Sukumara Varma, A

dc.contributor.author	Deepa, James
dc.contributor.author	Girija, D
dc.contributor.author	Sally K, Mathew
dc.contributor.author	Nazeem, P A
dc.contributor.author	Babu, T D
dc.contributor.author	Sukumara Varma, A
dc.date.accessioned	2018-08-04T05:33:48Z
dc.date.available	2018-08-04T05:33:48Z
dc.date.issued	2003
dc.identifier.citation	Journal of Tropical Agriculture 41 (1), 33-37.	en_US
dc.identifier.uri	http://hdl.handle.net/123456789/2099
dc.description.abstract	Nine strains of Ralstonia solanacearum (Smith) Yabuuchi et al. isolated from bacterial wilt affected plants of brinjal, chilli and tomato in three different agroclimatic zones of Kerala were compared based on the utilization of carbohydrates, hypersensitivity reaction on capsicum leaves and RAPD analysis. Among these, six isolates were grouped into Biovar III and three, into Biovar IIIA. The isolates belonged to Races 1 and 3. RAPD analysis with 10 decamer primers revealed a high degree of polymorphism among the isolates. The primer OPF 8 yielded a unique band of 1.45 kb size for Race 3. This could be considered as a marker for rapid identification of Race 3 isolates of R. solanacearum.	en_US
dc.language.iso	en	en_US
dc.publisher	Kerala Agricultural University, Vellanikara	en_US
dc.subject	Bacterial wilt; biovar; race; Ralstonia solanacearum; RAPD; solanaceous vegetables	en_US
dc.title	Detection of Ralstonia solanacearum race 3 causing bacterial wilt of solanaceous vegetables in Kerala, using random amplified polymorphic DNA (RAPD) analysis	en_US
dc.type	Article	en_US