Abstract:
Hi»h frequency somatic embryos were produced from hypocotyl cell suspension cultures
of Arufttis /i\f>ut>araL. (cvs. VR1-2 and TMV-7). Callus cultures were established on MS medium
augmented with B, vitamins, 2,4 D, NAA and K IN They were subsequently transferred to MS liquid
medium with same Mill-:, vitamins hut with different growth regulator combination and further
subcultured at two weeks intervals into fresh medium for embryo development Upon transfer to MS
liquid medium supplemental with growth regulators, more mini her of somatic embryos developed from
the omhryogenic cells. Different stages of embryos with varying frequencies were observed in cell
suspension cultures. The embryos were counted at each stage of development after one month of
culture. The 2,4-D addition was proved to he necessary to induce embryogenesis and embryo
maturation. Matured embryos were subsequently germinated and produced plant lets. Regenerate were
successfully transplanted to potting soil and grown to maturity ill the field.