Indirect somatic embryogensis and plantlet regeneration in groundnut (Arachishypogaea L.) through cell culture

Abstract

Hi»h frequency somatic embryos were produced from hypocotyl cell suspension cultures of Arufttis /i\f>ut>araL. (cvs. VR1-2 and TMV-7). Callus cultures were established on MS medium augmented with B, vitamins, 2,4 D, NAA and K IN They were subsequently transferred to MS liquid medium with same Mill-:, vitamins hut with different growth regulator combination and further subcultured at two weeks intervals into fresh medium for embryo development Upon transfer to MS liquid medium supplemental with growth regulators, more mini her of somatic embryos developed from the omhryogenic cells. Different stages of embryos with varying frequencies were observed in cell suspension cultures. The embryos were counted at each stage of development after one month of culture. The 2,4-D addition was proved to he necessary to induce embryogenesis and embryo maturation. Matured embryos were subsequently germinated and produced plant lets. Regenerate were successfully transplanted to potting soil and grown to maturity ill the field.